胰岛素样生长因子1、载脂蛋白酶E基因多态性与阿尔茨海默病关系的研究

发布时间：2018-03-09 14:52

本文选题：胰岛素样生长因子基因　切入点：载脂蛋白酶E基因　出处：《中风与神经疾病杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨河南省汉族人群胰岛素样生长因子1(insulin like growth factor 1,IGF-1)基因rs972936位点多态性、载脂蛋白酶E(Apo E)基因多态性与阿尔茨海默病(Alzheimer’s disease,AD)之间的相关性。方法选取58例AD患者和126例年龄、性别相匹配的健康对照(ND)者为研究对象,柱层析法提取外周血基因组DNA,采用PCR和基因测序技术检测IGF-1基因rs972936位点及Apo E基因型多态分布,并进行对比分析。结果与ND组比较,AD组IGF-1基因rs972936位点3种基因型分布总体差异有统计学意义(χ~2=6.108,P=0.047),其中AD组中GG基因型的频率高于对照组(70.7%51.6%,χ~2=5.935,P=0.015),G等位基因频率明显高于健康对照组(χ~2=6.502,P=0.011);AD组Apo Eε4等位基因频率可能增加AD的患病风险(OR=2.872,95%CI 1.542~5.351)(P=0.001);Apo Eε4等位基因不影响IGF-1基因rs972936位点的基因型或等位基因的分布频率(P0.05)。结论 IGF-1基因rs972936位点多态性与河南汉族人群AD的发病可能有相关性,其中GG基因型、G等位基因可能是AD发病的独立于Apo Eε4等位基因的危险因素。Apo Eε4等位基因是散发性AD的主要危险因素。
[Abstract]:Objective to investigate the association of rs972936 locus polymorphism of insulin-like growth factor 1insulin like growth factor 1 (IGF-1) gene, apolipoprotein enzyme (apo) gene polymorphism with Alzheimer's disease (AD) in Henan Han population. Methods 58 patients with AD and 126 patients with age were enrolled in this study. The genomic DNA of peripheral blood was extracted by column chromatography in healthy controls with sex matching. The rs972936 loci of IGF-1 gene and the polymorphism distribution of Apo E genotypes were detected by PCR and gene sequencing techniques. Results compared with ND group, the distribution of rs972936 loci of IGF-1 gene in AD group was significantly different from that in ND group (蠂 ~ 2 ~ (2) = 6.108). The frequency of GG genotype in AD group was higher than that in control group (70.7Q.6g), and the frequency of GG genotype in AD group was significantly higher than that in control group (70.7Q.6g). The frequency of rs972936 locus in AD group was significantly higher than that in healthy control group. The allele frequency of Apo E 蔚 4 may increase the risk of AD in the control group (蠂 2 + 6.502P = 0.011). Conclusion the polymorphism of rs972936 locus of IGF-1 gene and the frequency of distribution of IGF-1 gene rs972936 locus in Henan Province are not affected by the allele of Apo E 蔚 4. Conclusion the polymorphism of rs972936 locus of IGF-1 gene and the frequency of distribution of IGF-1 gene rs972936 locus in Henan Province are not affected by the allele of Apo E 蔚 4 in the control group. Conclusion the polymorphism of rs972936 locus of IGF-1 gene and the frequency of allele distribution in Henan Province are not affected by the allele of ApoE 蔚 4. The incidence of AD in Han nationality may be related. The GG genotype G allele may be a risk factor independent of Apo E 蔚 4 allele in AD. Apo E 蔚 4 allele is the main risk factor of sporadic AD.
【作者单位】：郑州大学第五附属医院神经内科;
【基金】：河南省科技厅科技攻关项目研究基金(112102310114)
【分类号】：R749.16

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