Aβ蛋白淀粉样变成分在正常人血小板膜的表达

发布时间：2018-03-20 01:36

本文选题：淀粉样变　切入点：血小板　出处：《中国老年学杂志》2016年14期 　论文类型：期刊论文

【摘要】：目的探讨正常人血小板中是否存在Aβ蛋白淀粉样变成分。方法筛选正常人群340例,采用Sysmex-200型血细胞分析仪测定血液血小板数量(PLT)、血小板分布宽度(PDW)、平均血小板体积(MPV)、血小板比容(PCT)和大型血小板比例(P-LCRs),进一步提纯血小板,将血小板分成两部分,一部分用于提取血小板膜蛋白,另一部分用硫磺素T(Th T)荧光探针检测血小板膜上淀粉样变。抗Aβ抗体检测淀粉样变前多肽的免疫反应。Western印迹观察Aβ蛋白分子聚合。结果 Th T荧光探针检测血小板几乎全部显示阳性反应。进一步抗Aβ免疫组织化学实验发现绿色荧光与Aβ抗体反应阳性部位重叠。SDS-PAGE分析血小板提取的蛋白成分含有大分子Aβ聚合物。结论正常人血小板中存在Aβ淀粉样变成分,可能参与AD患者淀粉样变的发生。
[Abstract]:Objective to investigate whether A 尾 -amyloidosis is present in the platelets of healthy people. The platelet count was determined by Sysmex-200 blood cell analyzer, the platelet distribution width was determined, the mean platelet volume, platelet specific volume and the ratio of large platelets were determined. The platelets were further purified, and the platelets were divided into two parts. Part of it is used to extract platelet membrane proteins, The other part was the detection of amyloidosis on platelet membrane with the fluorescence probe of sulfur Thunb Th Th. The immunoreaction of anti-A 尾 antibody in detecting amyloidosis prepeptide. Western blotting was used to observe the molecular polymerization of A 尾 protein. Almost all of the plates showed positive reaction. Further anti-A 尾 immunocytochemical assay found that the protein extracted from platelets contained macromolecular A 尾 polymer by superposition of green fluorescence and A 尾 antibody reaction. Conclusion\\\; There is A 尾 -amyloidosis in human platelets. It may be involved in the development of amyloidosis in AD patients.
【作者单位】：天津医科大学医学检验学院;天津检验检疫理化检验中心;
【基金】：国家自然科学基金(30973157)
【分类号】：R749.16;R446.11

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