姜黄素对阿尔茨海默病模型小鼠脑内胶质细胞介导的炎症反应的抑制作用

发布时间：2018-03-23 08:45

本文选题：阿尔茨海默病　切入点：姜黄素　出处：《国际神经病学神经外科学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨姜黄素对AD模型小鼠学习记忆能力的影响。方法将80小鼠按随机原则分四组,对照组、Alcl3组、溶剂组及姜黄素组。Alcl3制作小鼠AD模型,之后给予腹腔注射姜黄素3 d,给药后14 d水迷宫测试学习记忆能力。免疫染色及western blot方法检测Aβ42、GFAP和Iba-1β蛋白水平。ELISA方法检测海马区炎症因子IL-1β,IL-6和TNF-α的水平。结果与对照组比,Alcl3能够导致逃避潜伏期延长和记忆下降更明显,说明AD模型成功建立。与Alcl3组和溶剂治疗组相比,姜黄素组明显减少潜伏期及增加穿越次数,而且显著减少Aβ42表达。相比Alcl3组,姜黄素组的GFAP和Iba-1β以及海马区IL-1β、IL-6及TNF-α的指标下降有显著意义。结论研究表明姜黄素有潜在治疗AD的可能,通过下调Alcl致胶质细胞过度活化引起炎症反应,而提高学习记忆能力。
[Abstract]:Objective to investigate the effect of curcumin on learning and memory ability of AD model mice. Methods 80 mice were randomly divided into four groups: control group, solvent group and curcumin group. After intraperitoneal injection of curcumin for 3 days, the ability of learning and memory was tested by water maze on the 14th day after administration. The levels of A 尾 42G FAP and Iba-1 尾 protein and Iba-1 尾 protein were detected by immunocytochemistry and western blot method. Elisa was used to detect the levels of IL-1 尾 -guan6 and TNF- 伪 in hippocampal area. In the control group, the escape latency was prolonged and the memory decreased more significantly than that in the control group. Compared with Alcl3 group and solvent treatment group, curcumin group significantly reduced latency and increased traversal times, and significantly reduced A 尾 42 expression. Compared with Alcl3 group, curcumin group significantly decreased the expression of A 尾 42, compared with Alcl3 group, curcumin group significantly decreased the expression of A 尾 42. In curcumin group, the indexes of GFAP and Iba-1 尾 and IL-1 尾 IL-6 and TNF- 伪 in hippocampus decreased significantly. Conclusion curcumin has the potential to treat AD by down-regulating the inflammatory response of glial cells induced by Alcl and improving the ability of learning and memory.
【作者单位】：哈尔滨医科大学附属第四医院;黑龙江省护理高等专科学校;
【基金】：哈尔滨市科技创新人才研究专项资金(2013RFQYJ079) 黑龙江省教育厅(12531307)
【分类号】：R749.16

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