阿尔茨海默病患者淋巴细胞中双链RNA-依赖的蛋白激酶水平与认知障碍的相关性

发布时间：2018-03-24 08:45

本文选题：PKR　切入点：阿尔茨海默病　出处：《中风与神经疾病杂志》2017年08期

【摘要】：目的研究双链RNA-依赖的蛋白激酶(the double stranded RNA-dependent protein kinase,PKR)在阿尔茨海默病(Alzheimer’s disease,AD)患者外周血淋巴细胞(Peripheral lymphocytes,PBLs)中的表达及活性;探讨其水平及活性改变与AD患者认知障碍的相关性。方法选取AD、PD及健康老年人各20例,应用蛋白质免疫印迹技术检测PBLs中总PKR及磷酸化PKR(p-PKR)的表达,分析比较3组中PKR表达及活性的差异,并利用皮尔森相关分析探讨PKR表达及活性与AD、PD病程和病情的关系。结果与正常对照组相比,总PKR、p-PKR蛋白表达水平在AD患者的PBLs中均明显升高(P0.05),且总PKR的表达量与AD患者认知障碍严重程度呈正相关(r=0.799,P0.01);在PD组PBLs中总的PKR与p-PKR蛋白水平均升高(P0.05)。结论在AD患者中,PKR的异常表达及活化不仅体现在中枢神经系统,而且可在PBLs中检测到,其表达量与认知障碍严重程度成正相关,有可能成为AD早期诊断及病情评价的外周标记物。
[Abstract]:Objective to investigate the expression and activity of double-stranded RNA-dependent protein kinase (double stranded RNA-dependent protein kinase) in peripheral blood lymphocytes of patients with Alzheimer's disease (AD). Methods the expression of total PKR and phosphorylated PKRp-PKR in PBLs were detected by Western blotting technique. The expression and activity of PKR in the three groups were analyzed and compared, and the relationship between the expression and activity of PKR and the course of the disease and the disease state was discussed by Pearson correlation analysis. The expression of total PKRP-PKR protein was significantly increased in PBLs of AD patients, and the expression of total PKR was positively correlated with the severity of cognitive impairment in AD patients, and the total PKR and p-PKR protein levels in PBLs of PD group were significantly higher than that in AD patients. Conclusion in AD patients, there is a positive correlation between the expression of total PKR and the severity of cognitive impairment. Conclusion there is a positive correlation between the expression of total PKR and the severity of cognitive impairment in AD patients (P 0.05). The abnormal expression and activation of PKR are not only reflected in the central nervous system, but also in the central nervous system. Furthermore, it can be detected in PBLs that its expression level is positively correlated with the severity of cognitive impairment, and may be a peripheral marker for early diagnosis and evaluation of AD.
【作者单位】：河北医科大学第二医院神经内科河北省神经病学重点实验室;北京四季青医院神经内科;河北医科大学第二医院神经外科;
【基金】：河北省重点研发自筹项目(科技惠民工程)(No.162777151) 河北省自然科学基金资助项目(No.H2017206156)
【分类号】：R749.16

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