控制性阈值低血压对老年大鼠Tau和Aβ表达及术后认知影响

发布时间：2018-03-27 07:10

本文选题：低血压　切入点：脑脊液　出处：《实用医学杂志》2017年15期

【摘要】：目的探讨不同持续时间控制性阈值低血压对大鼠p-Tau-181和Aβ-42蛋白表达和认知的影响。方法健康清洁老年雄性SD大鼠39只,采用随机数字表法分为4组:C组为对照组(n=9);A1、A2、A3组(n=10)分别每日降压持续2、4、6 h,连续5 d,降压组维持平均动脉压(MAP)在50~55 mm Hg安全范围内。Morries水迷宫法检测大鼠的空间学习记忆能力,ELISA法检测脑脊液Aβ42蛋白和p-Tau-181蛋白水平。结果各组大鼠死亡情况无明显差异(P0.05);与C组比较,A2组和A3组逃避潜伏期延长和游泳距离增加(P0.05);术后3~7 d,大鼠脑脊液的p-Tau-181和Aβ42蛋白表达增加;随着控制性阈值低血压时间延长,与A1组相比,术后A2组和A3组逃避潜伏期延长和游泳距离增加(P0.05);A2组和A3组的脑脊液Aβ42和p-Tau-181显著增加(P0.05);与A2组比较,A3组Aβ42增加不明显,而p-Tau-181在术后第5天增加(P0.05)。结论长时间的控制性阈值低血压可引起术后认知功能障碍,可能与Aβ42和p-Tau-181蛋白表达的增加相关。
[Abstract]:Objective to investigate the effects of controlled threshold hypotension on the expression and cognition of p-Tau-181 and A 尾 -42 protein in rats. Four groups of rats were randomly divided into 4 groups: group C as control group (n = 9). Group A _ (1) A _ (2) A _ (2) A _ (3) group (n = 10). The blood pressure was lowered for 6 hours for 5 days, and the mean arterial pressure (MAPP) was maintained for 5 days.) the spatial learning of rats was detected by Morries water maze method within the safety range of 50,55mm Hg. The levels of A 尾 42 protein and p-Tau-181 protein in cerebrospinal fluid (CSF) were detected by amnesia Elisa. Results there was no significant difference in death rate of rats in each group (P 0.05); compared with group C, the escape latency and swimming distance of group A 2 and group A 3 were prolonged and swimming distance increased (P 0.05). The expression of p-Tau-181 and A 尾 42 protein were increased. With the prolongation of hypotension time of controlled threshold, compared with A1 group, the escape latency and swimming distance of A2 and A3 groups were prolonged and the cerebrospinal fluid A 尾 42 and p-Tau-181 increased significantly in group A 2 and group A 3, but the increase of A 尾 42 in group A 3 was not significant compared with that in group A 2. Conclusion prolonged controlled threshold hypotension may cause postoperative cognitive dysfunction, which may be related to the increased expression of A 尾 42 and p-Tau-181 protein.
【作者单位】：南昌大学第二附属医院;
【基金】：江西省科技厅基金(编号:20142BBG70067) 南昌大学第二附属医院青年基金(编号:2016YNQN12019)
【分类号】：R749.16

【相似文献】