阿魏酸对Aβ25-35诱导PC12细胞的保护机制研究

发布时间：2018-03-29 08:02

本文选题：阿尔兹海默病　切入点：阿魏酸　出处：《辽宁中医杂志》2015年01期

【摘要】：目的:观察阿魏酸对β-淀粉样蛋白25-35(βamyloid peptide25-35,Aβ25-35)诱导的阿尔兹海默病(Alzheimer disease,AD)模型细胞的抗氧化能力的影响,探讨当归防治AD的作用及机制。方法:用Aβ25-35诱导PC12细胞凋亡,以不同浓度阿魏酸对抗干预,以MTT比色法观测阿魏酸对AD模型细胞增殖的保护作用,以比色法检测阿魏酸对AD模型细胞培养液中超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)的影响。用RT-PCR法检测Apbb1,乙酰胆碱酯酶(Ache)基因的表达。结果:阿魏酸对损伤的PC12细胞的存活率有提高作用,阿魏酸在2 mmol/L时可明显降低模型细胞培养液中MDA、LDH含量,提高SOD活力,差异有统计学意义(P0.01)。阿魏酸可下调Apbb1和Ache的mRNA表达,差异有统计学意义(P0.01)。结论:阿魏酸对Aβ25-35损伤的PC12细胞有保护作用,机制可能与提高细胞抗氧化能力相关,与下调Apbb1、Ache的mRNA表达量相关。
[Abstract]:Aim: to observe the effect of ferulic acid on the antioxidant ability of 尾 -amyloid 25-35 (尾 -amyloid 25-35) -induced Alzheimer disease (AD) model cells, and to explore the effect and mechanism of Angelica sinensis on the prevention and treatment of AD. Methods: apoptosis of PC12 cells was induced by A 尾 25-35. The protective effect of ferulic acid on the proliferation of AD model cells was observed by MTT colorimetry. The effects of ferulic acid on superoxide dismutase (SOD), malondialdehyde (MDA) MDAD and lactate dehydrogenase (LDH) in the culture medium of AD model cells were detected by colorimetry. The expression of Apbb1, acetylcholinesterase (Achea) gene was detected by RT-PCR method. The survival rate of PC12 cells was increased. Ferulic acid could significantly decrease the content of MDA-LDH and increase the activity of SOD at 2 mmol/L. Ferulic acid could down-regulate the mRNA expression of Apbb1 and Ache. Conclusion: ferulic acid has protective effect on PC12 cells damaged by A 尾 25-35, the mechanism may be related to the increase of antioxidant ability of the cells, and the down-regulation of mRNA expression of Apbb1Ache.
【作者单位】：甘肃中医学院;甘肃省中医方药挖掘与创新转化重点实验室甘肃省中药新产品创制省级工程实验室;
【基金】：国家自然科学基金项目(81060284)
【分类号】：R749.16

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