小鼠脑组织中单胺类神经递质含量测定方法的建立与比较

发布时间：2018-03-30 12:44

本文选题：单胺类神经递质　切入点：液相色谱-紫外检测器　出处：《第二军医大学学报》2017年09期

【摘要】：目的比较高效液相色谱(HPLC)-紫外光(UV)、HPLC-荧光(FLD)和HPLC-质谱(MS)3种检测方法测定小鼠不同脑组织中单胺类神经递质含量的优劣性,并应用最优方法测定血管性抑郁小鼠不同脑组织中单胺类神经递质的含量。方法分别采用HPLC仪器搭配UV、FLD和MS检测器建立小鼠不同脑组织中多巴胺(DA)、去甲肾上腺素(NE)和5-羟色胺(5-HT)的含量测定方法,并进行系统的方法学验证,采用HPLC-FLD法对血管性抑郁小鼠不同脑组织中单胺类神经递质DA、NE和5-HT含量进行测定。结果 HPLC-UV法的定量限分别为DA 103.5ng/mL、NE 107.5ng/mL、5-HT 93.6ng/mL;HPLC-FLD法的定量限分别为DA 10.35ng/mL、NE 10.75ng/mL、5-HT9.36ng/mL;HPLC-MS法的定量限分别为DA 10.35ng/mL、NE 32.25ng/mL、5-HT 9.36ng/mL。HPLC-FLD和HPLC-MS法对DA和5-HT的测定优于HPLC-UV法,HPLC-FLD法对于NE的测定优于HPLC-MS法,而HPLC-MS法存在较强的基质效应。HPLC-FLD法检测结果示血管性抑郁小鼠海马组织中DA含量最低,大脑皮质中5-HT含量最低,而不同脑组织中NE含量没有明显差别。结论与HPLC-UV法和HPLC-MS法相比,HPLC-FLD法更适用于小鼠脑组织中单胺类神经递质(DA、NE、5-HT)的含量测定。DA和5-HT可以用作血管性抑郁症疾病的诊断标志物。
[Abstract]:Objective to compare the determination of monoamine neurotransmitters in different brain tissues of mice by high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) and HPLC- mass spectrometry (HPLC-MS) respectively.The contents of monoamine neurotransmitters in different brain tissues of vascular depression mice were determined by optimal method.Methods the methods for the determination of dopamine, noradrenaline (NE) and 5-hydroxytryptamine (5-HT) in different brain tissues of mice were established by HPLC and MS detector.The contents of monoamine neurotransmitter DAFNE and 5-HT in different brain tissues of vascular depression mice were determined by HPLC-FLD method.HPLC-FLD method showed that DA content was the lowest in hippocampus and 5-HT content in cerebral cortex was the lowest in vascular depression mice, but there was no significant difference in NE content in different brain tissues.Conclusion compared with HPLC-UV and HPLC-MS, HPLC-FLD is more suitable for the determination of monoamine neurotransmitters in brain tissue of mice. DA and 5-HT can be used as diagnostic markers of vascular depression.
【作者单位】：第二军医大学东方肝胆外科医院药材科;同济大学附属第一妇婴保健院药剂科;第二军医大学长海医院神经内科;第二军医大学长海医院药材科;
【基金】：国家自然科学基金(81571299) 军队医学创新工程(16CXZ012)~~
【分类号】：R749

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