阿尔茨海默病患者尿液细胞向诱导多能干细胞及神经细胞的诱导转化

发布时间：2018-03-31 21:02

本文选题：阿尔茨海默病　切入点：诱导多能干细胞　出处：《中国病理生理杂志》2015年03期

【摘要】：目的:获得阿尔茨海默病(Alzheimer disease,AD)患者来源的诱导多能干细胞(induced pluripotent stem cells,i PSCs)。方法:选取3例临床诊断明确的AD病例,收集病人尿液,分离出尿路上皮细胞,对所得细胞进行原代培养。利用电转染的方法将带有Oct4、Sox2、Klf4和SV40LT的质粒导入原代细胞内,将其重编程为i PS细胞。随后利用双向抑制Smad通路的方式继续诱导其神经分化。结果:AD病人尿液来源的细胞(以下称为尿液细胞)均成功诱导成i PS细胞,其诱导效率与正常人来源的细胞无明显差异。且病人的i PS细胞可成功分化为神经细胞,分化效率亦与正常人来源细胞相近。结论:阿尔茨海默症患者来源的尿液细胞可重编程为i PS细胞,所得到的i PS细胞可成功分化成有功能的神经元以及神经胶质细胞。
[Abstract]:Objective: to obtain pluripotent stem cells derived from patients with Alzheimer disease (AD).Methods: urinary epithelial cells were isolated from 3 patients with AD.By electrotransfection, the plasmids containing Oct4, Sox2, Klf4 and SV40LT were transferred into primary cells and reprogrammed as iPS cells.The neural differentiation was further induced by bidirectional inhibition of Smad pathway.Results all the urine derived cells (hereinafter referred to as urine cells) were successfully induced into iPS cells, and the induction efficiency was not significantly different from that of normal human cells.The iPS cells of the patients could be successfully differentiated into nerve cells, and the differentiation efficiency was similar to that of normal cells.Conclusion: urine cells from patients with Alzheimer's disease can be reprogrammed as iPS cells, and the derived iPS cells can be successfully differentiated into functional neurons and glial cells.
【作者单位】：中山大学附属第六医院神经科;中国科学院广州生物医药与健康研究院;
【基金】：广东省医学科学研究基金资助项目(No.A2012211) 天河区2014年度科技计划项目(No.201404kw028)
【分类号】：R749.16

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