重复肢体远隔缺血适应对慢性脑低灌注大鼠空间学习记忆的影响及机制

发布时间：2018-04-07 15:39

本文选题：慢性脑低灌注　切入点：肢体远隔缺血适应　出处：《山东医药》2017年24期

【摘要】：目的探讨重复肢体远隔缺血适应(LRIC)对慢性脑低灌注大鼠空间学习记忆的影响及作用机制。方法选择健康雄性SD大鼠60只,随机均分为假手术组、模型组、单次LRIC组、多次LRIC组,后三组采用双侧颈总动脉结扎(2VO)法建立大脑低灌注损伤模型,假手术组仅分离双侧劲总动脉不予结扎。术后3天时,单次LRIC组进行1次LRIC,具体方法为采用纱布条绑扎双侧下肢完全阻断股动脉血流,绑扎10 min,松开10 min,每次3个循环;多次LRIC组每天进行LRIC 1次,连续6周。LRIC结束后4周采用Morris水迷宫试验检测逃避潜伏期及目标象限时间百分比,激光多普勒血流仪检测海马CA1区脑血流,电镜观察海马CA1区轴索及血管的超微结构。结果假手术组、多次LRIC组逃避潜伏期均短于模型组和单次LRIC组,目标象限时间百分比均长于模型组和单次LRIC组,组间比较P0.05或0.01。假手术组、模型组、单次LRIC组、多次LRIC组海马CA1区相对脑血流分别为100%、(61.3±11.7)%、(59.5±13.1)%、(80.2±14.6)%,假手术组、多次LRIC组均高于模型组和单次LRIC组(P均0.05)。模型组海马CA1区血管内皮不光滑、基底膜增厚、周围明显水肿,轴索明显断裂、板层分离;多次LRIC组上述改变明显减轻。结论重复LRIC可改善慢性脑血流低灌注导致的认知功能障碍;增加海马区血供、抑制海马区微血管损伤可能是其作用机制。
[Abstract]:Objective to investigate the effect and mechanism of repeated limb remote ischemic adaptation (LRICC) on spatial learning and memory in rats with chronic cerebral hypoperfusion.Methods 60 male Sprague-Dawley rats were randomly divided into three groups: sham operation group, model group, single LRIC group, multiple LRIC group, and the latter three groups were treated with bilateral common carotid artery ligation.In the sham operation group, bilateral common arteries were separated and not ligated.On the 3rd day after operation, the single LRIC group was performed once. The specific method was to completely block the femoral artery blood flow in the lower extremities of both sides by using gauze bandage, ligation for 10 mins, release for 10 mins, and 3 cycles for each time, and LRIC was performed once a day in the LRIC group several times a day.The escape latency and the percentage of target quadrant time were measured by Morris water maze test, the cerebral blood flow in CA1 area of hippocampus was measured by laser Doppler flow analyzer, and the ultrastructure of axons and blood vessels in CA1 region of hippocampus were observed by electron microscope at 4 weeks after the end of LRIC.Results the escape latency of sham operation group, multiple LRIC group was shorter than that of model group and single LRIC group, and the percentage of target quadrant time was longer than that of model group and single LRIC group (P0.05 or 0.01).In the sham operation group, model group, single LRIC group, multiple LRIC group, the relative cerebral blood flow of the hippocampal CA1 area was 61.3 卤11.7 and 59.5 卤13.1 respectively. The sham operation group, multiple LRIC group were higher than the model group and the single LRIC group (P 0.05).In the model group, vascular endothelium was not smooth, basement membrane thickened, peripheral edema, axonal rupture and lamellar separation were observed in the hippocampal CA1 area, and the above changes were significantly alleviated in the multiple LRIC group.Conclusion repeated LRIC can improve the cognitive dysfunction induced by chronic cerebral blood flow hypoperfusion and increase the blood supply in the hippocampus and inhibit the microvascular injury in the hippocampus.
【作者单位】：潍坊医学院;首都医科大学低氧医学研究所;
【基金】：国家自然科学基金资助项目(81573867) 首都卫生发展科研专项资助项目(首发2011-1001-03)
【分类号】：R749.1

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