早期社会隔离对雌性ICR小鼠焦虑水平、社会行为及神经内分泌的影响

发布时间：2018-04-09 06:06

本文选题：隔离　切入点：应激　出处：《四川动物》2017年01期

【摘要】：许多证据表明早期生活应激会影响动物成年后的行为和神经化学改变,然而该过程的机制还不完全清楚。脑源性神经营养因子(BDNF)、催产素(OT)和糖皮质激素受体(GR)参与对应激的神经适应性反应。本实验将出生3 d后的雌性ICR小鼠幼仔每日隔离3 h直至出生21 d,在60日龄时检测其焦虑水平、社会行为及血清中皮质酮(CORT)和OT的水平,同时检测了中枢BDNF、OT和GR的阳性神经元表达。结果表明,与对照组相比,早期隔离组小鼠的焦虑水平增加,运动性和社会行为减少,同时血清中CORT水平上升、海马CA1和CA2/3区的BDNF及GR的阳性神经元表达下降,下丘脑室旁核和视上核的OT阳性神经元表达减少。这些结果表明,早期社会隔离下调了BDNF、OT和GR的水平,并通过调制应激系统影响雌性ICR小鼠的情绪与社会行为。
[Abstract]:There is much evidence that early life stress affects behavior and neurochemical changes in adult animals, but the mechanism of this process is not fully understood.Brain-derived neurotrophic factor (BDNF), oxytocin (OT) and glucocorticoid receptor (GRG) are involved in the neuroadaptive response to stress.The young female ICR mice were isolated for 3 hours daily until 21 days after birth. The anxiety level, social behavior, serum corticosterone (Cort) and OT levels were measured at the age of 60 days.The expression of BDNFOT and gr positive neurons was also detected.The results showed that compared with the control group, the anxiety level increased, the motor activity and social behavior decreased, the serum CORT level increased, and the expression of BDNF and gr positive neurons in hippocampal CA1 and CA2/3 decreased in the early isolation group.The expression of OT positive neurons in paraventricular nucleus and supraoptic nucleus of hypothalamus decreased.These results suggest that early social isolation down-regulated BDNFN OT and gr levels and affected the emotional and social behavior of female ICR mice by modulating stress system.
【作者单位】：北方民族大学生物科学与工程学院;
【基金】：国家自然科学基金项目(31260513;31460565) 北方民族大学研究生创新基金项目(YCX1646)
【分类号】：R749.72

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