TNF-α基因多态性与慢性精神分裂症的相关性研究

发布时间：2018-04-15 01:22

  本文选题：精神分裂症 + 肿瘤坏死因子　； 参考：《内蒙古医科大学》2012年硕士论文

【摘要】：目的探讨慢性精神分裂症的肿瘤坏死因子-α（TNF-α）基因启动子区的3个多态性位点，试图寻找到精神分裂症的患病易感基因，为精神分裂症的病因学研究提供一些理论上的依据。方法本研究采用的是病例-对照的关联研究，患者组共筛选出852例诊断为慢性精神分裂症的患者，正常对照组筛选出519例健康正常人。（1）运用PCR/RFLP(Polymerase Chain Reaction-restriction fragment length Polymorphism，聚合酶链式反应-限制性片段长度多态性)检测患者组和正常对照组血液样本中的TNF-α启动子区-G308A、-C863T和-T1031C这3个位点的基因型，，比较分析两组间的等位基因频率、基因型的分布和单倍型；2、使用SPSS13.0和UNPHASED3.1.4软件分析收集到的受试对象的数据。结果在基因型分布符合哈伯温伯格（Hardy-Weinberg）平衡（P0.05）的基础上：（1）患者组和正常对照组之间-G308A位点的基因型其分布频率及等位基因其频率未见统计学差异（P0.05）,患者组和正常对照组间的-C863A位点总体基因型频率有统计学差异（χ~2=8.288，P=0.016）,患者组和正常对照组-T1031C位点女性患者其基因型及其等位基因频率有统计学差异（分别为：χ~2=6.112，P=0.047；χ~2=4.54，P=0.033）；（2）总样本中rs1800629(A)-rs1800630(C)、rs1800630(C)-rs1799964(C)、rs1800629(G)-rs1800630(C)-rs1799964(T)、rs1800629(G)-rs1800630(C)-rs1799964(C)和rs1800629(G)-rs1800630(A)-rs1799964(T)的P值小于0.05。结论（1）慢性精神分裂症的TNF-a基因-C863A和-T1031C两位点基因型与其发病相关，但前者的相关性仅见于总体样本，后者的相关性仅见于总体样本和女性样本；（2）两组TNF-α基因的3个位点单倍型系统的分析结果如下：rs1800629(A)-rs1800630(C)、rs1800630(C)-rs1799964(C)、rs1800629(G)-rs1800630(C)-rs1799964(T)、rs1800629(G)-rs1800630(C)-rs1799964(C)和rs1800629(G)-rs1800630(A)-rs1799964(T)等,这些单倍型可能与精神分裂症这种疾病有相关性。
[Abstract]:Objective to explore three polymorphic loci in the promoter region of tumor necrosis factor- 伪 (TNF- 伪) gene in chronic schizophrenia, and to find out the susceptible gene of schizophrenia, and to provide some theoretical basis for the etiological study of schizophrenia.Methods the study was conducted in a case-control study in which 852 patients diagnosed as chronic schizophrenia were selected from the patient group.PCR/RFLP(Polymerase Chain Reaction-restriction fragment length Polymorphism, polymerase chain reaction-restriction fragment length polymorphism (RFLP) was used to detect TNF- 伪 promoter region G308An-C863T and T1031C in healthy controls.The genotype of the locus,The allele frequency, genotype distribution and haplotype 2 were compared between the two groups. The data were analyzed by SPSS13.0 and UNPHASED3.1.4 software.Results on the basis of genotypic distribution consistent with Hardy-Weinberger balance (P0.05)), there was no statistical difference in genotype frequency and allele frequency between patient group and normal control group (P 0.05), and between patient group and normal control group, there was no statistical difference in the distribution frequency and allele frequency of G308A locus.鐓х粍闂寸殑-C863A浣嶇偣鎬讳綋鍩哄洜鍨嬮�戠巼鏈夌粺璁″�﹀樊寮

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