朝医三神散防治血管性痴呆模型的研究

发布时间：2018-04-16 04:29

  本文选题：血管性痴呆 + Morris水迷宫　； 参考：《延边大学》2013年硕士论文

【摘要】：目的：观察朝医三神散(sanshensan,SSS)对血管性痴呆(Vascular Dementia, VD)模型大鼠血清中超氧化物歧化酶(supermxidedismutase, SOD)、丙二醛(malonaldchydc, MDA)、谷胱甘肽过氧化物酶(glulathione pcroxidase, GSH-PX)水平的影响,探讨其治疗血管性痴呆的疗效机制。方法：采用wistar系雄性大鼠42只,随机分为假手术组、手术组,手术组采用改良2-血管阻断法(2-VO)+硝普钠降压法制作VD模型,假手术组动物颈总动脉切开不夹闭。将VD模型大鼠随机分为假手术组、模型组、三神散大剂量组、三神散中剂量组、三神散小剂量组、喜得镇(Hyderginc)组,灌药各组给予不同的药物灌胃,假手术组及模型组给予等剂量的蒸馏水灌胃,连续灌胃28天,处死制取标本。观察VD模型大鼠学习记忆能力及血清中SOD、MDA、GSH-PX水平的变化,探讨VD的发病机制和三神散对VD模型大鼠缺血脑组织的保护作用。结果：1.与假手术组比较,模型组学习记忆能力明显降低(P0.05)。2.与模型组对照,各治疗组学习记忆能力明显改善(P0.05)。3.与假手术组比较,模型组血清SOD、GSH-PX含量明显降低,MDA含量明显升高(P0.05)。4.与模型组对照,各治疗组血清SOD、GSH-PX含量均明显升高(P＜0.01), MDA含量下降(P0.01)。5.与喜得镇组比较,三神散小剂量组SOD, GSH-PX, GSH含量明显降低(P＜0.05), MDA含量明显升高(P0.05),而三神散大、中剂量组SOD、GSH-PX、MDA含量差异不显著(P0.05)。结论：通过Morris水迷宫实验,观察和比较三神散对VD模型大鼠学习记忆能力的影响,说明三神散能改善VD大鼠学习记忆的能力。三神散可明显升高血管性痴呆模型大鼠血清中SOD、GSH-PX的活性,降低脂质氧化产物MDA的含量。
[Abstract]:Objective: to observe the effect of Sanshensan San (SSSs) on the levels of superoxide dismutase (SOD), superoxide dismutase (SOD), malondialdehyde (malonaldchydcc), glutathione peroxidase (Glutathione peroxidase) pcroxidase (GSH-PX) in the serum of vascular dementia rats, and to explore the mechanism of their therapeutic effect in the treatment of vascular dementia. Methods: the serum levels of superoxide dismutase (SOD), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (Glutathione peroxidase) (GSH-PX) in the serum of vascular dementia rats were observed.Methods: Forty-two male wistar rats were randomly divided into two groups: sham operation group, operation group, and modified 2-VOO sodium nitroprusside sodium hypotensive method to make VD model. In sham operation group, the common carotid artery was not clipped.VD rats were randomly divided into sham operation group, model group, large dose group, middle dose group, low dose group and Hyderginc group.Sham operation group and model group were given the same dose of distilled water.To observe the changes of learning and memory ability and the level of GSH-PX in serum of VD model rats, to explore the pathogenesis of VD and the protective effect of San Shen San on ischemic brain tissue of VD model rats.The result is 1: 1.Compared with sham operation group, the learning and memory ability of model group was significantly lower than that of sham operation group.Compared with the model group, the ability of learning and memory in each treatment group was significantly improved (P 0.05. 3).Compared with the sham-operated group, the serum level of GSH-PX in the model group decreased significantly and the MDA content increased significantly.Compared with the model group, the serum level of GSH-PX in each treatment group was significantly higher than that in the control group (P < 0.01), and the content of MDA was decreased (P < 0.01).Compared with Xidazen group, the content of SOD, GSH-PXX and GSH in the small dose group of Sanshen Powder decreased significantly (P < 0.05), and the content of MDA increased significantly (P < 0.05), while the content of MDA in the medium dose group was not significantly different from that in the medium dose group (P 0.05).Conclusion: the effects of Sanshen Powder on the learning and memory ability of VD rats were observed and compared by Morris water maze experiment, which indicated that San Shen San could improve the learning and memory ability of VD rats.Sanshen powder could increase the activity of SOD GSH-PX and decrease the content of lipid oxidation product MDA in the serum of vascular dementia rats.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.13
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本文编号：1757316