TNF-α-308基因多态性与强迫症的易感性研究

发布时间：2018-04-23 08:59

本文选题：肿瘤坏死因子α + 强迫症　；参考：《重庆医学》2014年04期

【摘要】：目的探讨TNF-α-308基因多态性与强迫症(OCD)易感性的关系。方法收集26例OCD患者及14例健康对照者的血液,提取DNA后采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)法测定TNF-α-308位点的基因多态性,进行各组间基因型分布和等位基因频率的χ2检验,比较各组间基因型分布的差异。计算等位基因的OR值和95%CI,以确定基因多态性与OCD易感性的关系。结果 OCD组及健康对照组均以TNF1/1占优势,OCD组为16.7%(TNF1/2)和83.3%(TNF1/1),而健康对照组中全为TNF1/1型。两组之间的基因型频率分布的比较,差异有统计学意义(χ2=15.73,P0.01)。OCD组与健康对照组的差异主要来自G等位基因,差异有统计学意义(P0.01),使其患OCD的危险性提高了1.93倍。结论 TNF-α-308基因多态性可能与OCD的易感性相关。
[Abstract]:Objective to investigate the relationship between TNF- 伪-308 gene polymorphism and OCD susceptibility. Methods the blood samples of 26 patients with OCD and 14 healthy controls were collected. The polymorphism of TNF- 伪 -308 locus was determined by polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) method after DNA was extracted. The genotype distribution and allele frequency of each group were tested by 蠂 2 test, and the difference of genotype distribution among groups was compared. The OR and 95 CI of alleles were calculated to determine the relationship between genetic polymorphism and susceptibility to OCD. Results in the OCD group and the healthy control group, TNF1/1 was dominant in the TNF1 / 2 group and 83.3 TNF1 / 1 / 1 in the OCD group, while in the healthy control group, the TNF1/1 type was found. The difference of genotype frequency distribution between the two groups was statistically significant (蠂 2 15.73% P0.01A. The difference between OCD group and healthy control group was mainly from G allele), the difference was statistically significant (P 0.01), which increased the risk of OCD by 1.93 times. Conclusion the polymorphism of TNF- 伪-308 gene may be associated with susceptibility to OCD.
【作者单位】：广东省中山市第三人民医院二科;重庆医科大学附属第一医院心理卫生中心;北京回龙观医院中心实验室;
【分类号】：R749.7

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