产前应激子代大鼠海马可塑性改变诱导抑郁样行为的发生

发布时间：2018-04-23 13:58

本文选题：产前应激 + 抑郁样行为　；参考：《科学技术与工程》2017年31期

【摘要】：研究旨在探索海马可塑性改变在产前应激(PS)子代大鼠抑郁样行为中的作用。通过建立大鼠产前束缚应激模型,采用强迫游泳实验检测子代大鼠的抑郁样行为,快速Golgi染色观察海马CA1区锥体细胞顶树突的树突棘密度,Western-blotting检测海马脑源性神经营养因子(BDNF)的表达。结果发现,与对照组相比较,PS组雌、雄子代大鼠强迫游泳实验(FST)中的不动时间均明显增加,有统计学差异(P0.05),而且PS组雌性子代大鼠的不动时间较雄性子代更加延长(P0.05)。PS组雌、雄子代大鼠海马CA1区锥体细胞的顶树突棘密度均较对照组显著减少(P0.05),PS组雌、雄子代之间无统计学差异。PS组雌、雄子代大鼠海马的BDNF表达均较对照组显著减少(P0.05),PS组雌、雄子代之间亦无显著性差异。这些结果说明海马CA1区锥体细胞的树突棘密度减少和海马BDNF表达减少参与PS导致的子代大鼠抑郁样行为的发生。
[Abstract]:The purpose of this study was to explore the role of hippocampal plasticity in depressive behavior of antepartum stress rats. By establishing a rat model of prenatal restraint stress, a forced swimming test was used to detect the depressive behavior of offspring rats. The expression of brain-derived neurotrophic factor (BDNF) in hippocampal CA1 was detected by Western-blotting. The results showed that the immobility time of female and male offspring of PS group was significantly longer than that of control group, and the immobility time of female offspring of PS group was longer than that of male offspring. The density of apical dendritic spine of hippocampal CA1 pyramidal cells in male offspring was significantly lower than that in control group. There was no significant difference between male offspring and male offspring. The expression of BDNF in hippocampus of male offspring was significantly lower than that of control group. There was no significant difference between male offspring. These results suggest that the decrease of dendritic spinous density and the decrease of hippocampal BDNF expression in the pyramidal cells of hippocampal CA1 region are involved in the occurrence of depressive behavior induced by PS in the offspring of rats.
【作者单位】：西安交通大学医学部法医学院;西安交通大学医学部基础医学院;
【基金】：国家自然科学基金青年项目(31200843) 陕西省自然科学基础研究计划(2016JM8041)资助
【分类号】：R-332;R749.4

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