丁苯酞对Aβ25-35诱导的阿尔茨海默病细胞模型的保护作用

发布时间：2018-04-24 03:24

本文选题：丁苯酞 + 阿尔茨海默病　；参考：《南方医科大学学报》2017年09期

【摘要】：目的研究丁苯酞对Aβ25-35诱导的AD细胞模型的保护作用及其作用机制。方法取对数生长期的N2a细胞,用20μmol/L Aβ25-35建立阿尔茨海默病细胞模型;实验分为AD组、丁苯酞组和对照组,其中丁苯酞组设立4个药物浓度梯度的亚组,分别为0.1、1、10、100μmol/L丁苯酞。MTT法检测细胞存活率;流式细胞仪检测细胞凋亡率;倒置相差显微镜下观察细胞形态学变化;q RT-PCR检测细胞TNF-α和IL-1β的mRNA含量。结果与对照组比较:AD组细胞存活率显著下降,细胞凋亡率明显增加,贴壁细胞数量明显减少,突起结构减少,TNF-α和IL-1β mRNA表达增高(P0.05);与AD组比较:丁苯酞组的细胞存活率提高,细胞凋亡率下降,贴壁细胞数量增多且结构较正常,TNF-α和IL-1β的mRNA表达减少(P0.05)。结论丁苯酞对Aβ25-35诱导的AD细胞模型具有保护作用,可能与抑制TNF-α和IL-1β等炎性因子的表达有关。
[Abstract]:Objective to study the protective effect of butyphthalide on AD cell model induced by A 尾 25-35 and its mechanism. Methods N2a cells of logarithmic growth stage were used to establish Alzheimer's disease cell model with 20 渭 mol/L A 尾 25-35, and were divided into AD group, butyphthalide group and control group, among which four drug concentration gradient subgroups were established in butyphthalide group. The cell survival rate was detected by MTT assay, the apoptosis rate was detected by flow cytometry, and the mRNA content of TNF- 伪 and IL-1 尾 was detected by inverted phase contrast microscope and Q RT-PCR. Results compared with the control group, the cell survival rate, the apoptosis rate, the number of adherent cells and the increased expression of TNF- 伪 and IL-1 尾 mRNA were significantly decreased in AD group, and the cell survival rate was increased in butyphthalide group as compared with that in AD group. The apoptosis rate decreased, the number of adherent cells increased and the mRNA expression of TNF- 伪 and IL-1 尾 decreased compared with normal cells. Conclusion butyphthalide has protective effect on AD cell model induced by A 尾 25-35, which may be related to the inhibition of the expression of inflammatory factors such as TNF- 伪 and IL-1 尾.
【作者单位】：中南大学湘雅三医院神经内科;
【基金】：湖南省科技厅重点研发计划(2016JC2052) 中南大学湘雅三医院新湘雅人才工程“至善领跑计划”(20150305)
【分类号】：R749.16

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