多奈哌齐与左卡尼汀治疗痴呆模型大鼠的疗效比较

发布时间：2018-04-27 16:14

  本文选题：阿尔茨海默病 + 左卡尼汀　； 参考：《中国老年学杂志》2017年08期

【摘要】：目的比较左卡尼汀和多奈哌齐对脑室注射链脲佐菌素(STZ)致痴呆大鼠的治疗作用。方法健康SD雄性大鼠32只,随机分为对照组、模型组、左卡尼汀组、多奈哌齐组(n=8)。模型组和治疗组在手术的第1、3天分别向两侧侧脑室内注射STZ 3 mg/kg,对照组给予相同体积的柠檬酸缓冲液。左卡尼汀组于手术10 d前给予左卡尼汀20 mg·kg~(-1)·d~(-1)灌胃,术后持续90 d,多奈哌齐组给予多奈哌齐0.45 mg·kg~(-1)·d~(-1)灌胃,其余两组给予等量生理盐水。术后第10、90天Morris水迷宫试验,观察大鼠学习记忆能力。术后8 w处死大鼠取海马送电镜观察神经元凋亡情况。结果术后10 d水迷宫测试,与对照组比较,模型组、左卡尼汀组、多奈哌齐组的潜伏期延长,过平台次数下降(P0.01)。术后治疗90 d,与模型组比较,多奈哌齐组的潜伏期缩短,过平台次数增加(P0.01),左卡尼汀组潜伏期缩短(P0.05),过平台次数无明显增加(P0.05)。左卡尼汀组与多奈哌齐组比较各指标差异有统计学意义(P0.01)。与模型组比较,两治疗组电镜下观察大鼠海马细胞结构细胞凋亡程度轻,以多奈哌齐治疗组明显。结论左卡尼汀与多奈哌齐都能减轻AD模型鼠海马神经元的凋亡,从而起到改善临床症状的作用,左卡尼汀效果不如多奈哌齐。
[Abstract]:Aim to compare the therapeutic effects of levacarnitine and Donepezil on dementia rats induced by intraventricular injection of streptozotocin (STZ). Methods Thirty-two healthy male SD rats were randomly divided into control group, model group, L-carnitine group and Donepezil group. The model group and the treatment group were injected with STZ 3 mg / kg on the 1st day of operation respectively. The control group was given the same volume of citric acid buffer. The levacarnitine group was given levacarnitine 20 mg / kg / d ~ (-1) before operation for 90 days after operation, and the Donepezil group was given Donepezil 0.45 mg / kg / d ~ (-1) / d ~ (-1). The other two groups were given the same amount of normal saline. The ability of learning and memory was observed by Morris water maze test on day 10 ~ 90 after operation. The rats were killed 8 weeks after operation to observe the apoptosis of neurons by electron microscope. Results compared with the control group, the latencies of model group, L-carnitine group and Donepezil group were prolonged and the times of platform passing were decreased (P0.01). 90 days after operation, compared with the model group, the latency of Donepezil group was shortened, the times of platform crossing was increased (P 0.01), the latency of leucarnitine group was shortened (P 0.05), and the frequency of crossing platform was not significantly increased (P 0.05). Compared with Donepezil group, there were significant differences in every index between Levocarnitine group and Donepezil group (P 0.01). Compared with the model group, the apoptotic degree of hippocampal cells in the treatment group was less than that in the Donepezil group under electron microscope. Conclusion both levacarnitine and Donepezil can reduce the apoptosis of hippocampal neurons in AD model rats and thus improve the clinical symptoms. The effect of levacarnitine is not as good as that of Donepezil.
【作者单位】： 哈尔滨医科大学附属第四医院神经内科;哈尔滨医科大学附属第四医院老年病科;
【基金】：黑龙江省自然基金资助项目(No.0200869)
【分类号】：R749.16;R-332
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本文编号：1811419