雷帕霉素对自闭症仔鼠神经、社交行为及海马区BDNF、Bcl-2蛋白表达的影响

发布时间：2018-04-27 17:53

本文选题：自闭症 + 雷帕霉素　；参考：《山东医药》2017年48期

【摘要】：目的探讨雷帕霉素对自闭症仔鼠神经、社交行为及海马区脑源性神经营养因子(BDNF)、Bcl-2蛋白表达的影响。方法将12只Wistar孕鼠分为对照组、丙戊酸钠组(模型组)和丙戊酸钠联合雷帕霉素组(观察组),每组4只。模型组和观察组妊娠12.5天时腹腔注射丙戊酸钠(600 mg/kg),对照组同期腹腔注射等量生理盐水;观察组腹腔注射丙戊酸钠(600 mg/kg)后,每天给予雷帕霉素(4 mg/kg)灌胃直至仔鼠断奶,每组仔鼠20只。仔鼠出生35天时,分别检测三组仔鼠神经行为(在中央区的活动时间和直立次数)、社交行为(社交行为时间及非社交行为时间)及海马区BDNF、Bcl-2表达。结果与对照组仔鼠比较,模型组及观察组仔鼠在中央区活动时间均增加,站立次数均减少;与模型组仔鼠比较,观察组仔鼠在中央区活动时间减少,站立次数增加;组间比较P均0.05。与对照组仔鼠比较,模型组及观察组仔鼠社交行为时间均减少,非社交行为时间均增加;与模型组仔鼠比较,观察组仔鼠社交行为时间增加,非社交行为时间减少;组间比较P均0.05。模型组仔鼠海马BDNF蛋白相对表达量低于对照组,Bcl-2蛋白相对表达量高于对照组;观察组仔鼠海马BDNF蛋白相对表达量高于模型组,Bcl-2蛋白相对表达量低于模型组;组间比较P均0.05。结论雷帕霉素可改善丙戊酸钠自闭症仔鼠模型神经行为表现,提高其社交能力;促进海马区BDNF表达、抑制Bcl-2表达,调节神经元异常发育、促进细胞凋亡可能是其作用机制。
[Abstract]:Objective to investigate the effects of rapamycin on neurologic, social behavior and brain-derived neurotrophic factor (BDNF) Bcl-2 protein expression in rats with autism. Methods Twelve pregnant Wistar rats were divided into control group, sodium valproate group (model group) and sodium valproate combined with rapamycin group (observation group, 4 rats in each group). The model group and the observation group were intraperitoneally injected with sodium valproate 600 mg / kg at 12.5 days of gestation, while the control group was intraperitoneally injected with the same amount of normal saline at the same time, and the observation group was treated with rapamycin 4 mg / kg daily after intraperitoneal injection of sodium valproate (600 mg / kg) until weaning. There were 20 rats in each group. At 35 days after birth, the expression of neurobehavioral (activity time and upright times in the central area), social behavior (social behavior time and non-social behavior time) and BDNFF-1 Bcl-2 expression in hippocampal area were measured in three groups. Results compared with the control group, both the model group and the observation group increased the activity time in the central area and decreased the standing times, and compared with the model group, the observation group decreased the activity time in the central area and increased the standing times. There was significant difference between the two groups (P < 0.05). Compared with the control group, the social behavior time of the model group and the observation group decreased, the non-social behavior time increased, and the observation group increased the social behavior time and decreased the non-social behavior time compared with the model group. There was significant difference between the two groups (P < 0.05). The relative expression of BDNF protein in hippocampus of the model group was lower than that of the control group, the relative expression of BDNF protein in the hippocampus of the observation group was higher than that of the model group, and the relative expression of Bcl-2 protein in the hippocampus of the observation group was lower than that of the model group (P < 0.05). Conclusion Rapamycin can improve the neurobehavioral behavior and social ability of rat model of sodium valproate, promote the expression of BDNF in hippocampus, inhibit the expression of Bcl-2, regulate the abnormal development of neurons, and promote apoptosis.
【作者单位】：唐山市妇幼保健院;
【分类号】：R749.94

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