脑还丹对快速老化小鼠SAMP8海马细胞内钙离子浓度及突触后致密区蛋白的影响

发布时间：2018-05-01 18:03

本文选题：脑还丹 + SAMP　；参考：《中华中医药杂志》2014年10期

【摘要】：目的:观察复方脑还丹对快速老化小鼠SAMP8海马区[Ca2+]i及突触后致密区PSD-95和Shank-1蛋白的影响。方法:将6月龄SAMP8小鼠(雄性)36只,随机分为模型组、脑还丹组和银可络组,每组各12只,SAMR1小鼠12只为正常对照组。治疗组分别以脑还丹浓缩液及银可络灌胃;正常对照组和模型组以同体积双蒸水灌胃,6周后进行行为学测试,测定各种小鼠海马组织细胞[Ca2+]i,采用免疫组化法和计算机图像分析技术测定海马CA1区PSD-95、Shank-1阳性神经元数目及光密度值。结果:Morris水迷宫实验:与模型组相比,正常对照组、脑还丹组和银可络组逃避潜伏期较模型组明显缩短(P0.05,P0.01),药物治疗组在原平台象限停留时间明显增加(P0.05,P0.001),且脑还丹组小鼠的表现显著优于银可络组小鼠(P0.05)。海马区[Ca2+]i:模型组较正常对照组海马细胞[Ca2+]i明显增高(P0.001);脑还丹治疗组及银可络治疗组[Ca2+]i较模型组明显降低(P0.001)。免疫组化实验:与模型组相比,正常组、脑还丹组及银可络组海马CA1区PSD-95、Shank-1阳性细胞数较之显著增多(P0.05,P0.01)。结论:脑还丹对快速老化小鼠SAMP8学习记忆功能具有改善作用,可能与稳定海马区细胞内[Ca2+]i,延缓细胞衰老,促进海马区PSD-95、Shank-1蛋白的表达,恢复细胞突触的结构和功能有关。
[Abstract]:Aim: to observe the effects of Fufang Naohuandan on [Ca2] I and PSD-95 and Shank-1 proteins in SAMP8 hippocampal area and postsynaptic compact area of rapidly aging mice. Methods: a total of 36 6-month-old SAMP8 mice (male) were randomly divided into three groups: model group, Naohuandan group and Yinkoluo group. 12 mice of SAMR1 in each group were as normal control group. In the treatment group, Naohuandan concentrated solution and Yinkoluo were perfused into the stomach, and the behavior of the normal control group and the model group were measured after 6 weeks of gastric perfusion with the same volume of double-steamed water. The number and optical density of PSD-95 Shank-1 positive neurons in hippocampal CA1 were determined by immunohistochemistry and computer image analysis. Results: compared with the model group, the normal control group, Compared with the model group, the escape latency of Naohuandan group and Yinkoluo group was significantly shorter than that of the model group, and the residence time of the drug treatment group in the original platform quadrant increased significantly, and the performance of the Naohuandan group was significantly better than that of the Yinkoluo group. The hippocampal [Ca2] I in the model group was significantly higher than that in the normal control group (P 0.001), and that in the Naohuandan treatment group and the Yinkoluo treatment group [Ca2] I was significantly lower than that in the model group. Immunohistochemistry: compared with the model group, the number of PSD-95 PSD-95 Shank-1 positive cells in the hippocampal CA1 in normal group, Naohuandan group and Yinkoluo group were significantly increased. Conclusion: Naohuandan can improve the learning and memory function of SAMP8 in rapidly aging mice, which may be related to stabilizing [Ca2] I in hippocampal cells, delaying cell senescence, promoting the expression of PSD-95 Shank-1 protein in hippocampus, and restoring the structure and function of synaptic cells.
【作者单位】：河南省人民医院;中山大学孙逸仙纪念医院;广东省中医院;
【基金】：广东省中医药局课题(No.2009155)~~
【分类号】：R749.16

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