海马髓鞘碱性蛋白表达的改变对大鼠抑郁样行为的影响

发布时间：2018-05-01 18:48

  本文选题：髓鞘碱性蛋白 + 抑郁症　； 参考：《广西医科大学》2013年硕士论文

【摘要】：抑郁症是危害人类身心健康的一种常见的精神类疾病。近年来,随着社会的进步,人们的生活节奏也越来越快,抑郁症的发病率也在不断呈现上升的趋势。 髓鞘碱性蛋白(myelin basic protein,MBP)是脊椎动物中枢神经系统(central nerve system, CNS)少突胶质细胞和周围神经系统雪旺细胞合成的一种弱碱性膜蛋白,含有多种碱性氨基酸。MBP是中枢神经系统(CNS)髓鞘的主要蛋白质,位于髓鞘浆膜面,维持CNS髓鞘结构和功能的稳定,具有神经组织特异性。 目的：探讨MBP蛋白表达的改变对抑郁大鼠的行为活动的影响 方法：大鼠随机分为正常组、模型组、MBP高表达组、RNA干扰组和空载体组。应用慢性不可预知温和应激刺激制备抑郁大鼠模型。应用强迫游泳实验,高架十字迷宫实验,旷场实验等行为学测试方法观察MBP对抑郁大鼠行为活动的影响。用免疫荧光观察MBP的表达,用免疫组化的方法观察MBP的分布,用Western Blot法检测MBP的含量。 结果： 1.MBP对CUS大鼠抑郁样行为的影响 从强迫游泳实验中可以看出,造模8周模型组大鼠的不动时间与正常组相比有显著性差异(P0.05)。诱导MBP表达后,MBP高表达组大鼠的不动时间比模型组大鼠的不动时间缩短,差异有统计学意义(P0.05)。空载体组和RNA干扰组相对于模型组来说无显著性差异。 从高架十字迷宫的实验中可以看出,造模8周模型组大鼠的开臂滞留时间百分比比正常组下降,差异有统计学意义(P0.05)。诱导MBP表达后,MBP高表达组大鼠的开臂滞留时间百分比比模型组延长,差异有统计学意义(P0.05)。空载体组和RNA干扰组相对于模型组来说无显著性差异。 在旷场实验中,从水平方向运动情况看,造模8周模型组大鼠的水平总位移比正常组大鼠下降,差异有统计学意义(P0.05)。诱导MBP表达后,MBP高表达组大鼠的水平总位移相对于模型组来说升高,差异有统计学意义(P0.05),但仍小于正常组。空载体组和RNA干扰组相对于模型组来说无显著性差异。从垂直方向的直立次数看,造模8周模型组大鼠的直立次数减少,与正常组比有显著性差异(P0.05)。诱导MBP表达后,MBP高表达组大鼠的直立次数相对于模型组来说增加,差异有统计学意义(P0.05),空载体组和RNA干扰组相对于模型组来说无显著性差异。 2.大鼠海马MBP的变化 免疫荧光中看出,荧光强度由高到低依次为MBP高表达组,空载体组,RNA干扰组。正常组和模型组中无荧光。 免疫组化实验看出MBP高表达组比模型组MBP蛋白表达要多,差异有统计学意义(P0.05),RNA干扰组比模型组MBP蛋白表达要少,差异有统计学意义(P0.05)。 Western Blot检测得出,MBP高表达组大鼠海马MBP蛋白含量显著高于模型组,差异有统计学意义(P0.05),RNA干扰组大鼠海马MBP蛋白含量显著低于高表达组,差异有统计学意义(P0.05)。 结论： 1.通过诱导MBP基因的改变可以引起其蛋白表达的改变 2.MBP蛋白表达水平的改变可以引起抑郁样行为的变化
[Abstract]:Depression is a common mental disease which endangers the physical and mental health of human beings. In recent years, with the progress of the society, people's life rhythm is getting faster and faster, and the incidence of depression is also increasing.
Myelin basic protein (MBP) is a weak alkaline membrane protein synthesized from the central nervous system of vertebrate (central nerve system, CNS) oligodendrocytes and peripheral nervous system Schwann cells. It contains a variety of basic amino acids.MBP is the main protein of the myelin sheath in the central nervous system (CNS). It is located in the myelin serous surface and is maintained. CNS myelin sheath is stable in structure and function.
Objective: To investigate the effect of MBP protein expression on behavioral behavior in depressed rats.
Methods: rats were randomly divided into normal group, model group, MBP high expression group, RNA interference group and no-load group. Chronic unpredictable mild stress stimulation was used to prepare depressive rats model. Forced swimming test, elevated cross maze test and open field test were used to observe the effect of MBP on behavior of depressive rats. The expression of MBP was observed by fluorescence. The distribution of MBP was observed by immunohistochemistry. The content of MBP was detected by Western Blot.
Result锛，

本文编号：1830626