血管性痴呆小鼠海马线粒体复合体活性变化

发布时间：2018-05-03 02:24

  本文选题：血管性痴呆 + 记忆　； 参考：《中国医院药学杂志》2015年16期

【摘要】：目的:探讨血管性痴呆(VD)小鼠海马线粒体复合体活性变化。方法:抽取并回输约40%总血量加双侧颈总动脉夹闭20 min建立VD模型。病理学研究检测神经元损伤;分光光度计法检测丙二醛(MDA)含量、锰超氧化物歧化酶(Mn-SOD)活性和线粒体复合体活性;Western-blot检测Mn-SOD蛋白表达;Morris水迷宫评估学习记忆能力。结果:与假手术组相比,模型组学习记忆能力显著降低(P0.05),MDA含量显著升高(P0.05),Mn-SOD活性和蛋白表达显著降低(P0.05);与假手术组相比,术后5 d模型组线粒体复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ活性显著降低(P0.05),术后15,30 d和60 d模型组线粒体复合体Ⅰ、Ⅱ活性无显著变化(P0.05),复合体Ⅲ、Ⅳ活性显著降低(P0.05);病理学检测显示模型组海马出现进行性神经元损伤。结论:线粒体复合体III、IV活性异常可能是VD的发病机制之一。
[Abstract]:Objective: to investigate the changes of mitochondrial complex activity in hippocampus of vascular dementia (VD) mice. Methods: the VD model was established by extracting and regurgitating 40% total blood volume and bilateral common carotid artery occlusion for 20 min. The neuronal damage was detected by pathology, the content of malondialdehyde (MDA), the activity of manganese superoxide dismutase (Mn-SOD) and the activity of mitochondrial complex (Mn-SOD) were detected by spectrophotometer, and the expression of Mn-SOD protein by Morris water maze was detected by Western-blot to evaluate the ability of learning and memory. Results: compared with the sham-operated group, the learning and memory ability of the model group was significantly lower than that of the sham-operated group, and the MDA content of the model group was significantly higher than that of the sham-operated group, and the activity and protein expression of P0.05Mn-SOD were significantly lower than that of the sham-operated group, and the mitochondrial complex 鈪，

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