精神分裂症circRNA的差异表达及其与症状的关系

发布时间：2018-05-03 05:36

  本文选题：circRNA表达水平 + 阴性症状　； 参考：《解放军医学杂志》2017年10期

【摘要】：目的探讨精神分裂症circRNA表达水平及其与患者阴性、阳性症状的关系。方法分别选取5例精神分裂症患者和正常人外周血进行基因芯片筛查,确定9种存在差异表达的circRNA,并在102例精神分裂症患者和103例正常人中进行PCR验证,进一步行精神分裂症阳性和阴性症状量表评估。结果精神分裂症患者外周血circRNA_102101、circRNA_102315、circRNA_104597、circRNA_101835、circRNA_101836的表达水平明显下调(P0.05或P0.01)。circRNA_103102、circRNA_103704的表达水平明显上调(P0.01)。circRNA_102101、circRNA_103102的ΔCt值与阳性症状呈明显正相关(P0.01或P0.05)。circRNA_103704与阳性症状、一般精神病理呈明显正相关(P0.01或P0.05)。思维障碍与circRNA_102101、circRNA_103102、circRNA_102315、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P0.01或P0.05)。激活性与circRNA_102101、circRNA_103102、circRNA_104597、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P0.01或P0.05)。偏执与circRNA_102101、circRNA_103102、ci rcRNA_103704、ci rcRNA_102802的ΔCt值呈明显正相关(P0.01或P0.05)。攻击性与ci rcRNA_102101、circRNA_103102、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P0.01或P0.05)。circRNA_103704进入阳性症状的回归方程(P0.01);circRNA_103704、circRNA_102315进入一般病理表现的回归方程(P0.01或P0.05)。结论circRNA_103704、circRNA_103102与精神分裂症阴性、阳性症状有明显关联,可能是精神分裂症病理过程主导的调控因子。
[Abstract]:Objective to investigate the expression of circRNA in schizophrenia and its relationship with negative and positive symptoms. Methods five patients with schizophrenia and 10 healthy controls were selected for gene microarray screening, and 9 differentially expressed circRNAs were identified. PCR was performed in 102 schizophrenic patients and 103 normal controls. The scale of positive and negative symptoms of schizophrenia was further evaluated. Results the expression level of circRNA102101 circRNA1022315 circRNA104597 circRNA101836 in peripheral blood of schizophrenic patients was significantly down-regulated, P0.01n.circRNA103102circRNA103704 or P0.01circRNA102circRNA103704 was significantly up-regulated. The 螖 Ct value of 102101circRNA103102 was positively correlated with the positive symptoms (P0.01 or P0.05).circRNA_103704), and the general psychopathology was positively correlated with the symptoms (P0.01 or P0.05). There was a significant positive correlation between thinking disorder and circRNA102101 circRNAs, 103102 circRNAs, 102315 circRNAs, 103704 circRNA102802, P0.01 or P0.05. Activation was positively correlated with the 螖 Ct value of circRNAs 1031021 and circRNAs 103102 / circRNAs in 103704 circRNAs, and the 螖 Ct values of circRNA102802 were positively correlated with the number of circRNAs. The 螖 Ct value of 103102ci rcRNA103704 rcRNA_102802 was positively correlated with the 螖 Ct value of 102101 circRNAs (P0.01 or P0.05). There was a significant positive correlation between the 螖 Ct values of the tip103704 circRNA102802 and the ci rcRNA102101 circRNAs. There was a significant positive correlation between the 螖 Ct values of P0.01 or P0.05).circRNA_103704 entering the positive symptoms regression equation. P0.01 or P0.05. the number of circRNAs 103704 circRNA102315 entered the regression equation P0.01 or P0.05 for the general pathological manifestations. Conclusion\\\
【作者单位】： 解放军102医院全军心理疾病防治中心;
【基金】：南京军区医学科技创新课题(12MA002)~~
【分类号】：R749.3
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本文编号：1837230