盐酸美金刚干预对血管性痴呆大鼠海马脑源性神经营养因子的表达及氧化应激水平的影响

发布时间：2018-05-04 07:25

本文选题：盐酸美金刚 + 血管性痴呆　；参考：《中国老年学杂志》2014年04期

【摘要】：目的观察盐酸美金刚干预对血管性痴呆(VD)大鼠海马区脑源性神经营养因子(BDNF)表达以及氧化应激水平的影响。方法选取60只健康雄性Wistar大鼠为研究对象,随机分为模型组,对照组,观察组,每组各20只。模型组、观察组均采用持久双侧颈总动脉结扎方法制作血管性痴呆大鼠模型,对照组不做结扎处理,术后8 w模型组给予羟甲基纤维素钠干预,观察组给予盐酸美金刚干预,连续4 w后对各组大鼠进行水迷宫试验,然后处死,检测并比较3组大鼠脑海马区BDNF表达及氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)水平的差异,分析盐酸美金刚的干预效果。结果对照组水迷宫测试时间为(92.8±37.6)s,模型组为(154.6±38.7)s,观察组为(117.4±34.2)s,3组间差异具有统计学意义(P0.05)。对照组大鼠海马区神经元细胞正常,模型组海马区神经元细胞数量降低,观察组海马区形态学变化介于对照组与模型组之间。经病理图像分析软件分析以及SOD、MDA的光密度(OD)值测定,3组大鼠海马区BDNF平均灰度值以及MDA、SOD表达水平有显著差异(P0.05)。结论盐酸美金刚可清除海马区氧自由基,减轻组织损伤,并上调海马区BDNF的表达保护神经元细胞。
[Abstract]:Objective to observe the effect of MJ on the expression of brain-derived neurotrophic factor (BDNF) and the level of oxidative stress in hippocampus of rats with vascular dementia (VD). Methods 60 healthy male Wistar rats were randomly divided into three groups: model group, control group and observation group. The model group and the observation group were all treated with permanent bilateral common carotid artery ligation method to make vascular dementia rats. The control group was not treated with ligation, the model group was treated with sodium hydroxymethyl cellulose at 8 weeks after operation, and the observation group was treated with methadone hydrochloride. After 4 weeks, the rats in each group were tested by water maze test, and then killed. The expression of BDNF and the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the hippocampus of the three groups were detected and compared. Results the test time of water maze in the control group was 92.8 卤37.6 s, that in the model group was 154.6 卤38.7 s, and that in the observation group was 117.4 卤34.2 Ms. The difference was statistically significant (P 0.05). The hippocampal neurons in the control group were normal and the number of neurons in the hippocampal area in the model group was decreased. The morphological changes of the hippocampal area in the observation group were between the control group and the model group. There were significant differences in the mean gray value of BDNF and the expression level of BDNF in hippocampal area of the three groups by the analysis of pathological image analysis software and the optical density and ODV value of SDD. Conclusion MJ can scavenge the oxygen free radicals in the hippocampus, reduce tissue damage, and up-regulate the expression of BDNF in the hippocampal area to protect the neuronal cells.
【作者单位】：郑州大学第一附属医院;
【分类号】：R965;R749.1

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