出生后营养干预对宫内发育迟缓大鼠学习记忆的影响

发布时间：2018-05-05 19:54

本文选题：宫内发育迟缓 + 营养干预　；参考：《首都医科大学学报》2017年06期

【摘要】：目的研究营养干预对宫内发育迟缓(intrauterine growth retardation,IUGR)大鼠学习记忆的影响,探讨阿尔茨海默病(Alzheimer's disease,AD)等神经变性病可能的发育起源及营养相关机制。方法应用热量限制法制备IUGR大鼠模型。对出生后的对照大鼠(control,C)和IUGR大鼠(I)在哺乳期和断乳后分别给予自由取食标准饲料(ad libitum food intake,A)、限制取食70%标准饲料(restricted food intake,R)及自由取食高脂饲料(high fat food intake,H)处理,得到6组大鼠:对照自由取食(control with ad libitum food intake,CA)组、对照限制取食(control with restricted food intake,CR)组、对照自由取食高脂(control with high fat food intake,CH)组、IUGR自由取食(IUGR with ad libitum food intake,IA)组、IUGR限制取食(IUGR with restricted food intake,IR)组和IGRU自由取食高脂(IUGR with high fat food intake,IH)组。于新生、断乳和成年3个不同时期取材,比较各组大鼠体质量、脑质量、脑/体质量比、血糖、血三酰甘油和总胆固醇等指标的变化。应用Morris水迷宫实验,比较各组成年大鼠空间学习记忆能力的变化。结果新生IUGR大鼠的血糖和总胆固醇浓度与对照组相比降低,差异有统计学意义(P0.05)。断乳期,血三酰甘油在IA和IH组较正常出生体质量组升高(P0.05);血总胆固醇在CR组低于CA组(P0.05),CH、IA和IH组高于CR组(P0.05),IR组低于IA组(P0.05)。成年大鼠血总胆固醇浓度在CH、IA和IH组均高于CA组(P0.05)。断乳大鼠体质量,CH组高于CA组(P0.05),CR、IA和IR组均低于CA组(P0.05);脑质量,CR、IA、IH、IR组均低于CA组(P0.05);脑/体质量比在CR和IR组高于CA组(P0.05),CH组低于CA组(P0.05)。成年大鼠的脑、体质量变化与断乳时期相似。Morris水迷宫实验中,撤除平台后,CH及IR两组大鼠穿过平台的次数均多于CA组(P0.05);CH组大鼠在平台象限停留时间多于CA、CR和IR组,差异有统计学意义(P0.05)。结论出生后的不同营养干预可造成IUGR大鼠血糖、血脂等血液生物化学指标的变化,伴脑、体质量的差异及成年后空间学习记忆的改变,提示AD等神经变性病的脑内变化可能起源于生命早期,并受到生后营养状况的影响。
[Abstract]:Objective to study the effects of nutritional intervention on learning and memory in intrauterine growth retard rats, and to explore the possible developmental origin and nutrition-related mechanism of neuropathic diseases such as Alzheimer's disease (AD). Methods IUGR rat model was established by caloric restriction method. During lactation and after weaning, the control rats and IUGR rats were treated with free standard feed (ad libitum food intakea), restricted food in taking 70% standard diet and high fat food in take-over (high fat food in takeoff), respectively, during lactation and after weaning. Six groups of rats were obtained: the control with ad libitum food taken in CAA group, the control with restricted food in CRA group, the control with restricted food in the control group, and the control group in the control group, and the control with restricted food in the control group, and the control group in the control group. Compared with the control with high fat food group, the IUGR with ad libitum food in the IUGR-restricted intake group and the IGRU group in the IGRU group were free to eat the IUGR with high fat food in the IUGR group and the IGRU group in the control group. The control group was compared with the control group and the IGRU group in the group of IUGR with ad libitum food in the control group and the IGRU group in the group of IUGR with restricted food in the control group and the group of the IGRU group in the group of IUGR-IUGR with high fat food in the IGRU group. The changes of body weight, brain mass, brain mass ratio, blood glucose, triglyceride and total cholesterol were compared at three different stages of birth, weaning and adulthood. Morris water maze test was used to compare the spatial learning and memory ability of adult rats in each group. Results the concentrations of blood glucose and total cholesterol in neonatal IUGR rats were significantly lower than those in control group (P 0.05). During the weaning period, serum triglyceride was higher in IA and IH group than in normal birth weight group, and total serum cholesterol in CR group was lower than that in CA group (P 0.05) and in IH group was higher than that in CR group (P 0.05) and IR group (P 0.05) lower than that in IA group (P 0.05). The serum total cholesterol concentration of adult rats in CHIA and IH groups was higher than that in CA group (P 0.05). The body weight of the weaned rats in the Ch group was higher than that in the CA group (P 0.05) and in the IR group was lower than that in the CA group (P 0.05), the brain mass ratio in the CR and IR group was lower than that in the CA group (P 0.05), and the brain / body weight ratio in the CR and IR groups was higher than that in the CA group (P 0.05CH). The changes of brain and body mass in adult rats were similar to those in weaning period. In Morris water maze test, the rats of group Ch and IR had more times of passing through the platform than those of group CA (P 0.05). The duration of staying in the platform quadrant of rats in group C was longer than that in group Cacr and group IR. The difference was statistically significant (P 0.05). Conclusion different nutritional interventions after birth can cause changes in blood biochemical indexes such as blood glucose, blood lipid, brain and body mass, and spatial learning and memory in adult IUGR rats. The results suggest that the brain changes of neurodegenerative venereal diseases such as AD may originate from early life and be affected by postnatal nutritional status.
【作者单位】：首都医科大学燕京医学院生化与分子生物学教研室;首都医科大学宣武医院中心实验室;
【基金】：北京市自然科学基金(7132033) 首都医科大学基础-临床合作基金(16JL14) 燕京医学院科研基金(15QD02,15QD08,17QD05)~~
【分类号】：R-332;R749.16

【相似文献】