FPS-ZM1对糖基化终产物诱导海马区Aβ代谢水平的影响

发布时间：2018-05-07 14:22

本文选题：高级糖基化终末产物 + 阿尔兹海默病　；参考：《山东大学学报(医学版)》2014年07期

【摘要】：目的观察RAGE受体阻断剂FPS-ZM1对高级糖基化终产物(AGEs)诱导海马区Aβ及相关代谢因素表达水平的影响。方法将40只大鼠随机分为生理盐水组(NC组)、FPS-ZM1对照组、AGEs组和FPS-ZM1组。向大鼠海马区注射AGEs,以建立AGEs脑损伤模型,并用FPS-ZM1干预。造模3周后HE染色观察海马区神经元病理改变;Morris水迷宫实验检测逃逸潜伏期(EL);蛋白印迹法检测各组RAGE、p-NF-κB、BACE1和APP的蛋白表达;酶联免疫吸附法检测各组Aβ1-40、Aβ1-42的水平。结果与NC组、FPS-ZM1对照组相比,AGEs组及FPS-ZM1组海马神经元排列疏松,p-NF-κB、BACE1和APP的蛋白表达和Aβ1-40、Aβ1-42浓度均升高(P0.01);但FPS-ZM1组上述改变均明显低于AGEs组,同时AGEs组RAGE表达明显增强,EL显著延长(P0.01),但FPS-ZM1组与NC组和FPS-ZM1对照组相比无统计学差异(P0.05)。结论 RAGE受体阻断剂FPS-ZM1能通过降低Aβ及相关代谢因素的表达水平,有效抑制AGEs所致的脑损伤。
[Abstract]:Aim to investigate the effect of RAGE receptor blocker FPS-ZM1 on the expression of A 尾 and related metabolic factors in hippocampus induced by advanced glycation end product (ages). Methods Forty rats were randomly divided into normal saline group (NC group), FPS-ZM1 control group (ages group) and FPS-ZM1 group. AGEs brain injury model was established by injecting ages into hippocampus of rats, and FPS-ZM1 was used to intervene. The pathological changes of hippocampal neurons were observed by HE staining after 3 weeks. The escape latency was detected by Morris water maze test, the protein expression of RAGEP-NF- 魏 BnBACE1 and APP was detected by Western blot, and the levels of A 尾 1-40 A 尾 1-42 were detected by Elisa. Results the protein expression of p-NF- 魏 B, BACE1 and APP and the concentration of A 尾 1-40 A 尾 1-42 in hippocampal neurons in ages group and FPS-ZM1 group were significantly higher than those in NC group (P 0.01), but the above changes in FPS-ZM1 group were significantly lower than those in AGEs group. At the same time, the expression of RAGE was significantly increased in AGEs group, but there was no significant difference between FPS-ZM1 group and NC group and FPS-ZM1 control group. Conclusion FPS-ZM1, a RAGE receptor blocker, can effectively inhibit the brain injury induced by AGEs by reducing the expression of A 尾 and related metabolic factors.
【作者单位】：山东大学附属省立医院老年神经科;
【基金】：国家自然科学基金(30971036) 山东省自然科学基金(Y2008C13)
【分类号】：R749.16

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