衰老模型大鼠空间记忆的改变及抑郁样行为的研究

发布时间：2018-05-11 17:30

本文选题：衰老 + 学习记忆　；参考：《中国医科大学学报》2017年09期

【摘要】：目的建立一种大鼠衰老模型,观察其认知障碍和抑郁样行为的变化,并初步探讨该模型引发的2种疾病的共病机制。方法 SD雄性大鼠经D-半乳糖和三氯化铝联合给药后,采用Morris水迷宫测定其学习记忆能力,采用强迫游泳实验检测抑郁样行为的改变,采用高尔基染色观察海马区神经元的发育情况,检测超氧化物歧化酶(SOD)和丙二醛(MDA)含量,并分析氧化应激指标的改变。结果与对照组相比,模型组大鼠在水迷宫中逃避潜伏期延长(P0.05),平台停留时间和经过平台的次数减少(P0.05),强迫游泳实验中在水中的不动时间明显延长(P0.01),高尔基染色显示模型组大鼠海马区树突棘密度明显降低(P0.01),海马区SOD活力下降(P0.05),MDA含量增加(P0.05)。结论建立了一种新的并发认知障碍和抑郁的动物模型,这2种疾病的共病机制可能与自由基超载和代谢失衡过程中神经系统生长发育遭到破坏有关。
[Abstract]:Objective to establish a rat model of aging and to observe the changes of cognitive impairment and depressive behavior, and to explore the mechanism of co-disease induced by the model. Methods male SD rats were treated with Dgalactose and aluminum trichloride, and their learning and memory abilities were measured by Morris water maze, and depressive behavior was detected by forced swimming test. The development of hippocampal neurons was observed by Golgi staining. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) were detected, and the changes of oxidative stress indexes were analyzed. Results compared with the control group, In the model group, the escape latency of rats in the water maze prolonged P0.05, the time of staying on the platform and the times of passing through the platform decreased P0.05, and the immobility time in the water of forced swimming was significantly prolonged. Golgi staining showed the hippocampal area of the model group. The density of dendritic spine decreased significantly, and the activity of SOD decreased in hippocampus. Conclusion A new animal model of cognitive impairment and depression was established. The co-pathogenesis of these two diseases may be related to the damage of nervous system growth and development during the process of free radical overload and metabolic imbalance.
【作者单位】：沈阳医学院基础医学院病理生理学教研室;
【分类号】：R-332;R749.16

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