血管性痴呆大鼠海马神经元核因子-κB与环氧合酶-2水平的动态变化

发布时间：2018-05-19 05:19

本文选题：血管性痴呆 + 核因子κB　；参考：《中国老年学杂志》2014年02期

【摘要】：目的研究核因子(NF)-κB与环氧合酶(COX)-2在血管性痴呆(VD)大鼠海马的动态变化。方法将实验大鼠随机分为假手术组,全脑缺血再灌注30 min、2、6、24、48、7 d、15 d组。采用大鼠全脑缺血再灌注VD模型,Morris水迷宫检测空间学习记忆能力、HE染色观察大鼠海马神经元形态及数目、免疫组织化学染色法检测海马神经元NF-κB蛋白表达、Western印迹检测COX-2蛋白表达。结果与假手术组比较,VD组大鼠寻台潜伏期显著增加(P0.05),海马神经元凋亡30 min开始出现(P0.05),7 d达到峰值(P0.01)。NF-κB蛋白表达30 min开始升高,24 h达到峰值,15 d仍明显高于假手术组(P0.01)。COX-2蛋白表达2 h开始升高,7 d达到峰值,15 d仍高于假手术组(P0.01)。结论 NF-κB活化可能上调VD大鼠海马神经元中COX-2表达水平,进而参与脑损伤病理生理过程的调节。
[Abstract]:Objective to study the dynamic changes of NF-魏 B and cyclooxygenase-2 in the hippocampus of vascular dementia rats. Methods the experimental rats were randomly divided into sham-operation group and global cerebral ischemia-reperfusion group for 30 minutes. The morphology and number of hippocampal neurons in rats were observed by using Morris water maze to detect the spatial learning and memory ability and the number of hippocampal neurons by HE staining. The expression of NF- 魏 B protein in hippocampal neurons was detected by immunohistochemical staining. Western blot was used to detect the expression of NF- 魏 B protein. Results compared with the sham operation group, the latency of stage finding in VD group increased significantly, and the hippocampal neuronal apoptosis began to appear at 30 min. The expression of P0.01 and NF- 魏 B protein reached the peak value on the 7th day. The expression of NF- 魏 B protein increased at 24 h and reached the peak value at 15 days after sham-operation. It was still significantly higher than that in the sham operation group. The expression of P0.01C. COX-2 protein increased at 2 h and reached the peak at 7 d. The expression of COX-2 protein was still higher than that in sham operation group at 15 d. Conclusion the activation of NF- 魏 B may up-regulate the expression of COX-2 in hippocampal neurons of VD rats, and then participate in the regulation of pathophysiological process of brain injury.
【作者单位】：重庆医科大学药理教研室;重庆医科大学生物化学与分子药理学重点实验室;
【基金】：国家自然科学基金资助项目(No.81100905)
【分类号】：R749.16

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