米诺环素对慢性脑低灌注大鼠海马神经元β位点剪切酶1和β淀粉样蛋白表达的影响

发布时间：2018-05-22 08:29

本文选题：米诺环素 + 海马　；参考：《中华老年心脑血管病杂志》2014年11期

【摘要】：目的观察不同剂量米诺环素对慢性脑低灌注大鼠海马神经元β位点剪切酶1(BACE1)和β淀粉样蛋白(Aβ)表达的影响。方法选择SD大鼠144只,随机分为假手术组、对照组、慢性脑低灌注组(模型组)、米诺环素低剂量组、中剂量组、高剂量组,每组24只。采用双侧颈总动脉永久结扎建立大鼠慢性脑低灌注模型,低、中、高剂量组在慢性脑低灌注模型的基础上分别给予5mg/(kg·d)、50mg/(kg·d)、500mg/(kg·d)的米诺环素连续灌胃。观察时间点分别为造模后1、2、3个月,采用免疫组织化学法检测海马CA1区神经元BACE1和Aβ表达。结果模型组1、2、3个月BACE1和Aβ表达较假手术组、对照组、低剂量组、中剂量组、高剂量组明显增加(P0.05)。高剂量组2、3个月BACE1表达较低剂量组明显降低[(1.40±1.77)个/视野vs(10.50±4.83)个/视野,(1.25±1.24)个/视野vs(12.35±2.57)个/视野,P0.05],3个月BACE1表达较中剂量组明显降低[(1.25±1.24)个/视野vs(12.00±1.02)个/视野,P0.05]。结论米诺环素能抑制慢性脑低灌注大鼠海马神经元BACE-1和Aβ表达,高剂量米诺环素对BACE-1抑制作用明显。
[Abstract]:Objective to observe the effects of different doses of minocycline on the expression of 尾 -locus shear enzyme (尾 -locus shear enzyme 1) and 尾 -amyloid protein A 尾 (尾 -amyloid) in hippocampal neurons of rats with chronic cerebral hypoperfusion. Methods 144 SD rats were randomly divided into sham operation group, control group and chronic cerebral hypoperfusion group (model group, minocycline low dose group, middle dose group, high dose group, 24 rats in each group). The rat model of chronic cerebral hypoperfusion was established by permanent ligation of bilateral common carotid artery. The low, middle and high dose groups were treated with minocycline 50 mg / kg / d (500 mg / kg / d) on the basis of chronic cerebral hypoperfusion model, respectively. The expression of BACE1 and A 尾 in hippocampal CA1 neurons were detected by immunohistochemical method at 1 and 3 months after the model. Results the expression of BACE1 and A 尾 in model group was significantly higher than that in sham operation group, control group, low dose group, middle dose group and high dose group. The expression of BACE1 in the high-dose group was significantly lower than that in the low-dose group [1.40 卤1.77 / vs(10.50 卤4.83] / visual field (1.25 卤1.24) / vs(12.35 卤2.57 / visual field (P0.05), and the expression of BACE1 in 3 months was significantly lower than that in the middle dose group [1.25 卤1.24 / vs(12.00 卤1.02]. Conclusion minocycline can inhibit the expression of BACE-1 and A 尾 in hippocampal neurons of rats with chronic cerebral hypoperfusion. High dose minocycline can inhibit the expression of BACE-1.
【作者单位】：遵义医学院附属医院神经内科;遵义医学院附属医院病理科;
【基金】：贵州省科学技术基金(2007-2217) 贵州省卫生厅科技基金(gzwkj2010-1-050)
【分类号】：R749.1

【参考文献】