杏花雨对血管性痴呆大鼠行为及其海马Bcl-2，Bax蛋白表达的影响

发布时间：2018-05-25 03:30

本文选题：血管性痴呆 + 杏花雨　；参考：《河北大学》2012年硕士论文

【摘要】：目的：本研究通过观察高纯度银杏黄酮注射制剂杏花雨对缺血再灌注造成的血管性痴呆(VD)大鼠行为及其海马Bcl-2，Bax蛋白表达的影响，探讨杏花雨对VD大鼠学习记忆能力的影响及可能的作用机制，旨在为该药防治VD提供实验依据。方法：采用双侧颈总动脉缺血再灌注+硝普钠降压法制作VD模型，模型复制成功的VD大鼠随机分为模型组、杏花雨A组、杏花雨B组、金纳多组，另设假手术组。术后第2天开始腹腔注射给药，剂量均为5mL/kg，给药时间共计21天，从给药第3周开始采用Morris水迷宫实验装置及动态图像采集分析系统，由计算机记录各种参数记录。水迷宫实验完成后第2天，各组分别迅速断头取脑组织做HE染色，观察大鼠大脑海马区形态学改变并做免疫组化检测大鼠海马CA1区Bcl-2、Bax蛋白表达。结果：1.水迷宫实验结果：从水迷宫训练第1天起，各组大鼠逃避潜伏期与假手术组比较没有差别（P0.05）；而第2天各组逃避潜伏期均明显缩短，模型组与各治疗组之间有明显差别（P0.05）；第3天开始成绩比较稳定，与模型组比较，杏花雨B与假手术组逃避潜伏期显著缩短（P0.01），，杏花雨A组与金纳多组逃避潜伏期明显缩短（P0.05）；第4至6天各组与模型组比较均有极显著差异（P0.01）；另外，与金纳多组相比，杏花雨B组逃避潜伏期短，差异有统计学意义（P0.05），杏花雨A组逃避潜伏期较短，但差异无统计学意义（P0.05）；2.病理学改变：假手术组大鼠海马CA1区未见明显病理变化。模型组大鼠海马CA1区神经元大量变性、死亡，呈现深红色染色，核固缩、深染。杏花雨A、B组、金纳多组与模型组相比，海马CA1区神经元胞体缩小，但形状与正常接近，变性坏死明显较轻。而3组之间以杏花雨B组大鼠海马CA1区形态与假手术组更接近。3.Bcl-2、Bax蛋白表达：假手术组大鼠海马CA1区可见少量Bcl-2，Bax阳性细胞表达，模型组Bcl-2，Bax阳性细胞数量明显增加（P0.01），其中Bax变化更为显著，Bcl-2/Bax比值较假手术组显著降低（P0.05）；经杏花雨A、B及金纳多治疗后，各治疗组大鼠海马CA1区Bcl-2阳性细胞数均有提高（P0.05），杏花雨B组有明显提高（P0.01）；大鼠海马CA1区Bax阳性细胞数降低，尤其以杏花雨B组降低明显（P0.01），Bax与Bcl-2的比值也较模型组明显提高（P0.05）。结论： 1.银杏黄酮可显著改善VD大鼠认知水平，提高学习记忆功能能力，同等剂量下，以杏花雨B效果最佳，杏花雨A与金纳多效果相当。 2.通过大鼠大脑组织海马CA1区病理形态学观察，证实银杏黄酮能保护VD大鼠海马区的CA1区神经细胞，以杏花雨B组效果最佳。 3.免疫组化结果证实银杏黄酮通过调节VD大鼠海马组织Bcl-2与Bax的蛋白表达，保护海马组织神经元，从而减轻脑缺血再灌注对海马神经元的损伤。其中杏花雨B的这种保护作用最强，杏花雨A与金纳多具有等效性。
[Abstract]:Objective: to observe the effect of high purity ginkgo flavone injection preparation apricot rain on the behavior of vascular dementia rats induced by ischemia and reperfusion and the expression of Bcl-2 Bax protein in hippocampus. To explore the effect of apricot rain on learning and memory ability of VD rats and its possible mechanism in order to provide experimental evidence for the prevention and treatment of VD. Methods: VD model was made by bilateral common carotid artery ischemia reperfusion with sodium nitroprusside. VD rats were randomly divided into three groups: model group, apricot flower rain group A, apricot flower rain group B, Jinnardo group and sham operation group. On the second day after operation, the dosage was 5 mL / kg, and the administration time was 21 days. From the third week of administration, the Morris water maze experimental device and the dynamic image collection and analysis system were used to record the parameters by computer. On the second day after the completion of the water maze experiment, the brain tissues of each group were quickly severed for HE staining. Morphological changes in the hippocampus of the rats were observed and the expression of Bcl-2P Bax protein in the CA1 area of the rat hippocampus was detected by immunohistochemistry. The result is 1: 1. The results of water maze experiment showed that from the first day of water maze training, the escape latency of rats in each group was not different from that of sham operation group (P 0.05), but on the second day, the escape latency of each group was significantly shorter than that of sham operation group. There were significant differences between the model group and the treatment group (P 0.05), and the results of the model group were stable at the beginning of the third day, compared with the model group. The escape latency of apricot flower rain B and sham operation group was significantly shortened (P 0.01), and the escape latency of apricot flower rain group A and Jinnadu group was significantly shortened (P 0.05); on the 4th to 6th day, there was a significant difference between each group and model group (P 0.01); in addition, compared with Jinnaduo group, the escape latency of apricot flower rain group A and Jinnaduo group was significantly shorter than that of Jinnaduo group. The escape latency of group B was shorter than that of group A, but there was no significant difference between group A and group A (P 0.05). Pathological changes: there were no obvious pathological changes in CA1 area of hippocampus in sham operation group. In the model group, the neurons in the hippocampal CA1 area were denatured and died, showing dark red staining, nuclear pyknosis and deep staining. Compared with the model group, the neuronal bodies in the CA1 area of the apricot rain group and Jinnaduo group were smaller, but the shape was close to the normal, and the degeneration and necrosis of the neurons were lighter than that of the model group. The morphology of CA1 in hippocampus of group B was closer to that of sham-operated group. 3. The expression of Bcl-2nBax protein was observed in the CA1 area of hippocampus of rats in sham-operation group, and a small number of Bcl-2mBax positive cells were found in the CA1 area of rats in sham-operation group. In the model group, the number of Bcl-2nBax positive cells increased significantly, and the ratio of Bcl-2 / Bax to Bcl-2 / Bax in the model group was significantly lower than that in the sham-operated group. In each treatment group, the number of Bcl-2 positive cells in the CA1 region of hippocampus was increased, and the number of Bax positive cells in the CA1 region of the hippocampus was decreased in group B, especially in the group B, the ratio of P0.01-Bax to Bcl-2 in group B was also significantly higher than that in the model group. Conclusion: 1. Ginkgo flavonoids could significantly improve the cognitive level and the learning and memory ability of VD rats. At the same dosage, the effect of apricot flower rain B was the best, and the effect of apricot flower rain A was the same as that of Jinnadio. 2. The pathomorphological observation of hippocampal CA1 area in rat brain tissue showed that ginkgo flavonoids could protect CA1 neurons in hippocampus of VD rats. The best effect was in group B of apricot rain. 3. The results of immunohistochemistry showed that ginkgo flavone could protect hippocampal neurons by regulating the expression of Bcl-2 and Bax in hippocampus of VD rats, thereby reducing the injury of hippocampal neurons induced by cerebral ischemia reperfusion. The protective effect of apricot flower rain B is the strongest, and apricot flower rain A is equivalent to Ginado.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R749.1

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