基因的多态性及其交互作用对儿童注意缺陷多动障碍的影响

发布时间：2018-05-29 21:39

本文选题：基因 + 注意力缺陷障碍伴多动　；参考：《中国学校卫生》2014年03期

【摘要】：目的探讨5-HTTLPR、5-HT2A受体基因(5-HTR2A)T102C多态性、多巴胺β羟化酶基因(DβH)5'侧翼区-1021C/T多态性及其交互作用对儿童注意缺陷多动障碍(ADHD)的影响,为ADHD儿童的干预与治疗提供理论依据。方法对包头市某小学确诊为ADHD的78例儿童和87名对照组儿童应用PCR-限制性片段长度多态性(PCR-RFLP)方法检测5-HTTLPR、5-HTR2A的T102C多态性及DβH5'侧翼区-1021C/T多态性,采用χ2检验分析3种基因多态性对ADHD的影响,运用Logistic回归分析基因-基因交互作用。结果 5-HTR2A的T102C的基因型频率在ADHD组与对照组间差异有统计学意义(χ2=5.28,P=0.02),5-HTTLPR、DβH5'侧翼区-1021C/T的基因型频率在两组间差异无统计学意义(χ2值分别为0.24,0.01,P值均0.05);5-HTR2A的T102C基因位点的突变型发生儿童ADHD的危险性是野生型的2.17倍,5-HTR2A的T102C基因位点的突变型分别与5-HTTLPR的S等位基因DβH5'侧翼区-1021C/T基因位点的突变型、共同作用发生儿童ADHD的危险性是单一野生型的2.00,2.80倍。结论 5-HTR2A的T102C的基因型频率在ADHD组与对照组间差异有统计学意义,5-HTTLPR、5-HTR2A的T102C多态性及DβH5'侧翼区-1021C/T多态性在儿童ADHD的发生中存在交互作用。
[Abstract]:Objective to investigate the effects of 5-HTTLPR-5-HT2A receptor gene (5-HTR2At102C) polymorphism, dopamine 尾 -hydroxylase gene (D 尾 Hf5'flanking region) polymorphism and its interaction on ADHDD in children with attention deficit hyperactivity disorder (ADHDD), and to provide a theoretical basis for intervention and treatment of children with attention deficit hyperactivity disorder (ADHDD). Methods T102C polymorphism of 5-HTTLPR-5-HTR2A and -1021C / T polymorphism in flanking region of D 尾 H 5'were detected by PCR- restriction fragment length polymorphism (PCR-RFLP) in 78 children diagnosed as ADHD in a primary school in Baotou city and 87 children in control group. 蠂 2 test was used to analyze the effect of three gene polymorphisms on ADHD, and Logistic regression analysis was used to analyze the gene-gene interaction. Results the genotype frequency of T102C in 5-HTR2A was significantly different between ADHD group and control group (蠂 ~ 2 / 5.28) (蠂 ~ 2 / 5 ~ (5.28) -1021C / T genotype frequency of 5-HTTLPRD 尾 H _ 5'flanking region had no significant difference between the two groups (蠂 ~ 2 ~ 2 = 0.240.01 P = 0.05 ~ (5-HTR2A) mutation type of T102C locus of T102C gene locus of 5-HTR2A. The risk of occurrence of ADHD in children was 2.17 times higher than that in T102C locus of wild type 2.17 times and that in S allele D 尾 H 5'flanking region of 5-HTTLPR was 1021C / T, respectively. The risk of ADHD in children with co-action was 2.00% and 2.80 times higher than that of single wild type. Conclusion the genotype frequency of T102C of 5-HTR2A is significantly different between ADHD group and control group. The T102C polymorphism of 5-HTTLPRP 5-HTR2A and the flanking region of D 尾 H 5'-1021C / T polymorphism have interaction in the occurrence of ADHD in children.
【作者单位】：包头医学院公共卫生学院;
【基金】：内蒙古自然科学基金项目(2010MS1110)
【分类号】：R749.94

【参考文献】