3种拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异

发布时间：2018-06-02 01:15

  本文选题：拟痴呆模型 + Morris水迷宫试验　； 参考：《医学研究生学报》2014年07期

【摘要】：目的 Morris水迷宫实验是一种评价空间学习记忆能力的经典方法,是评价痴呆动物模型复制结果的客观指标。近年来痴呆动物模型已成为研究老年性痴呆的重要手段。文中评价3种常见拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异。方法分别使用SD大鼠、昆明种小鼠以及快速老化小鼠制备Aβ脑室注射致痴呆模型、东莨菪碱腹腔注射致记忆障碍模型、快速老化模型。采用Morris水迷宫行为学测试方法,将这3种模型通过与其相应的对照组比较,考察其行为学差异。结果东莨菪碱模型与快速老化模型逃避潜伏期时间短于相应对照组,其中以东莨菪碱模型差异尤为显著[(35.47±3.78)s vs(50.61±3.94)s,P0.01];快速老化组模型与对照组差异有统计学意义[(17.39±4.71)s vs(33.79±7.81)s,P0.05];Aβ致痴呆模型与其对照组比较,模型定位航行实验成绩差异无统计学意义(P0.05)。结论东莨菪碱腹腔注射致记忆障碍模型较其他2种常用模型更为理想。Morris水迷宫行为学测试方法是一种评价拟痴呆动物的理想方法。
[Abstract]:Objective Morris water maze test is a classical method to evaluate spatial learning and memory ability, and it is an objective index to evaluate the replicating results of dementia animal models. In recent years, animal models of dementia have become an important means to study Alzheimer's disease. The differences of learning and memory behaviors of three common dementia animal models in Morris water maze behavior test were evaluated in this paper. Methods SD rats, Kunming mice and rapid aging mice were used to establish dementia model induced by A 尾 intracerebroventricular injection, amnesia induced by scopolamine intraperitoneal injection, and rapid aging model. The behavioral differences of the three models were compared with those of the control group by Morris water maze test method. Results the escape latency of scopolamine model and rapid aging model was shorter than that of control group. There were significant differences in scopolamine model (35.47 卤3.78s vs(50.61 卤3.94 vs(50.61 P0.01) and rapid aging group (17.39 卤4.71s vs(33.79 卤7.81 vs(33.79 卤7.81g / kg) compared with control group (P 0.05). Conclusion the amnesia model induced by scopolamine intraperitoneal injection is more ideal than the other two models. Morris water maze is an ideal method to evaluate dementia animals.
【作者单位】： 成都中医药大学临床医学院;
【基金】：国家自然科学基金(81303283) 四川省科技创新苗子工程资助项目(20132054)
【分类号】：R749.16
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本文编号：1966617