茶氨酸对抑郁模型大鼠行为学、神经递质的影响

发布时间：2018-06-04 06:40

本文选题：抑郁模型大鼠 + 茶氨酸　；参考：《中南大学》2013年博士论文

【摘要】：目的：观察茶叶提取物茶氨酸及氟西汀对抑郁模型大鼠行为学及前额叶皮质(PFC)、伏隔核(NAC)、纹状体(ST)、杏仁核(AM)和海马(HIP)等边缘系统—皮层—纹状体—苍白球—丘脑(LCSPT)回路相关脑区单胺递质及谷氨酸递质的影响,探讨茶氨酸可能的抗抑郁机制。研究方法： 1.动物模型：选取雄性SD大鼠66只,随机分为模型组(17只)、氟西汀组(17只)、茶氨酸组(17只)、对照组(15只),前三组采用连续21天慢性轻度不可预见性应激(CUMS)结合孤养的方法建立慢性抑郁大鼠模型,茶氨酸组和氟西汀组分别给予2mg/kg/Qd茶氨酸和1.54mg/kg/Qd氟西汀灌胃21天,模型组和对照组给予同体积溶剂灌胃21天。 2.行为学指标：实验第1天,第22天,各组取9只大鼠,记录实验过程中各组大鼠的体重、旷场试验的活动总路程、糖水偏爱试验的糖水偏爱值、和强迫游泳试验的不动时间,研究茶氨酸及氟西汀对上述指标的影响。 3.神经递质指标：实验第22天,各组取8只大鼠(对照组6只),处死大鼠,取出PFC、NAC、ST、AM和HIP等脑组织,用库仑阵列电化学高效液相色谱法测定各组抑郁模型大鼠上述脑区单胺递质(NE、5-HT、HIP)的浓度及其代谢产物和谷氨酸(GLU)递质及谷氨酰胺(GLN)的浓度。结果： 1.与对照组相比,模型组大鼠反应迟钝,体重增加减慢,在规定时间内自主水平活动减少,糖水消耗量、糖水偏爱百分比降低,强迫游泳试验的不动时间增长(P0.05)。与模型组相比,茶氨酸组和氟西汀组大鼠体重增加速度正常、自主运动能力增强,糖水消耗量、糖水偏爱百分比显著增加,强迫游泳试验的不动时间减少(P0.05)。 2.与对照组相比,模型组大鼠PFC、NAC、ST (NE阴性)、AM (NE、5-HT阴性)、HIP等脑区单胺神经递质NE、DA和5-HT及部分代谢产物的浓度明显降低,GLU(GLN)浓度明显升高(P0.05)。 3.与模型组相比,氟西汀组PFC、NAC、ST (NE阴性)、AM(NE\5HT阴性)、HIP(NE阴性)等脑区单胺神经递质浓度及部分代谢产物的浓度显著升高,GLU (GLN)的浓度显著降低(P0.05)。与模型组相比,茶氨酸组PFC、NAC、ST (NE阴性)、AM(5HT阴性)、HIP等脑区单胺神经递质浓度及部分代谢产物的浓度显著升高,GLU (GLN)的浓度显著降低(P0.05) 结论： 1.抑郁模型大鼠各项行为学指标异常,前额叶、伏隔核、海马、纹状体(NE阴性)、杏仁核(NE、5-HT阴性)等脑区NE、5-HT、 DA浓度下降,GLU浓度上升,表明抑郁模型大鼠存在抑郁行为和不同脑区神经递质改变,说明模型建立成功。 2.茶氨酸和氟西汀均可以有效改善抑郁模型大鼠的抑郁行为,并提高PFC、NAC、HIP、AM、ST等脑区单胺神经递质NE、5-HT、 DA及部分代谢产物的浓度,降低GLU及其前体GLN的浓度,说明茶氨酸抗抑郁作用与可能机制和氟西汀类似。
[Abstract]:Objective: Effects of tea extract theanine and fluoxetine on behavior and LCSPTs in prefrontal cortex of rats with depression were observed, such as PFCN, NACN, STT, AMAM and HIPP), such as cortex-striatum, globus pallidus and thalamus (LCSPTs). Effects of monoamine and glutamate transmitters in Guannao region, To explore the possible antidepressant mechanism of theanine. Research methods: 1. Animal models: 66 male SD rats were selected, The rats were randomly divided into three groups: model group (n = 17), fluoxetine group (n = 17), theanine group (n = 17) and control group (n = 15). Theanine group and fluoxetine group were given 2mg/kg/Qd theanine and 1.54mg/kg/Qd fluoxetine for 21 days, and the model group and control group were given the same volume solvent for 21 days. 2. Behavioral indicators: on day 1 and day 22, 9 rats were taken from each group. The body weight, the total distance of open field test, the sugar water preference value of sugar water preference test, and the immobility time of forced swimming test were recorded. To study the effects of theanine and fluoxetine on these indexes. 3. Neurotransmitter index: on the 22nd day of the experiment, 8 rats in each group (6 rats in the control group) were killed, and the brain tissues such as HIP and HIP were taken out. Coulomb array electrochemical high performance liquid chromatography (HPLC) was used to determine the concentration of monoamine transmitter NE5-HT HIPs, its metabolites, glutamate GLU transmitters and glutamine (GLN) in the brain of rats with depression. Results: 1. Compared with the control group, the rats in the model group were retarded in reaction, weight gain slowed down, the activity of their own level decreased, the consumption of sugar water, the percentage of preference for sugar water decreased, and the immobility time of forced swimming test increased by 0.05%. Compared with the model group, theanine group and fluoxetine group showed normal weight gain rate, increased spontaneous exercise ability, increased sugar water consumption, percentage of sugar water preference, and reduced the immobility time of forced swimming test (P 0.05). 2. Compared with the control group, the concentration of monoamine neurotransmitters NEDA, 5-HT and some metabolites in the brain regions of the model group were significantly lower than those in the control group, and the concentration of GLU GLN in the brain regions of the model group was significantly higher than that of the control group, and the concentration of the monoamine neurotransmitters NEDA, 5-HT and some metabolites in the brain regions of the model group was significantly higher than that of the control group. 3. Compared with the model group, the concentration of monoamine neurotransmitters and some metabolites in fluoxetine group was significantly higher than that in the model group. The concentration of monoamine neurotransmitters and some metabolites in fluoxetine group was significantly higher than that in fluoxetine group. Compared with the model group, the concentration of monoamine neurotransmitters and some metabolites in the brain regions of the PFCnAC-NE-negative and AM5HT negative brain regions were significantly higher than that of the model group, and the concentration of GLU (GLN) decreased significantly (P 0.05). Conclusion: 1. The behavioral parameters of depression model rats were abnormal, the brain regions of prefrontal lobe, nucleus accumbens, hippocampus, striatum were negative for NE, and the amygdala was negative for NE-5HT), but the concentration of DA decreased and GLU increased. The results showed that there were depressive behaviors and neurotransmitter changes in different brain regions in the depression model rats, which indicated that the model was established successfully. 2. Theanine and fluoxetine can effectively improve the depressive behavior of rats with depression, and increase the concentration of monoamine neurotransmitter NE5-HT, DA and some metabolites in the brain, such as PFCnAC-HIPAMST, and decrease the concentration of GLU and its precursor GLN. This suggests that theanine has a similar antidepressant effect to the possible mechanism and fluoxetine.
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R749.4;R-332

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