CREB磷酸化水平在慢性应激模型大鼠中表达研究

发布时间：2018-06-08 08:29

本文选题：环磷酸腺苷反应元件结合蛋白 + 慢性应激　；参考：《中国神经精神疾病杂志》2014年01期

【摘要】：目的探讨慢性应激状态下,以及持续给予盐酸氟西汀后,大鼠海马及前额叶部位环磷酸腺苷反应元件结合蛋白(cAMP-response element binding protein,CREB)水平和磷酸化CREB(p-CREB)表达的变化。方法成年雄性Sprague-Dawley(SD)大鼠50只随机分为三组:应激组26只,药物组12只,对照组12只。应激组与药物组大鼠均经过8周慢性应激刺激,药物组在第1周开始给予盐酸氟西汀(10mg/g,I.P.),直到8周末。8周后行糖水偏好实验和旷场实验,根据8周末糖水偏爱率将应激组大鼠分为应激敏感组和应激适应组。应激结束后将四组大鼠断头处死,采用蛋白免疫印记技术检测大鼠海马、额叶部位CREB和p-CREB水平。结果 8周末应激敏感组糖水偏爱率低于应激适应组、药物组及对照组(均P0.05);应激敏感组的跨格次数、中心格时间和直立次数低于药物组和对照组(均P0.05),与应激适应组无统计学差异(均P0.05)。应激敏感组海马及前额叶中p-CREB水平明显低于应激适应组、药物组及对照组(均P0.05);四组大鼠海马部位、前额叶CREB表达水平无明显差异。结论海马和额叶部位CREB磷酸化水平可能与应激导致的情绪障碍发生机制相关。
[Abstract]:Objective to investigate the level of cyclic adenosine monophosphate (camp) response element binding protein (cAMP-response element binding protein) and the expression of phosphorylated CREB-p-CREBin in hippocampus and prefrontal lobe of rats under chronic stress and after continuous administration of fluoxetine hydrochloride. Methods 50 adult male Sprague-Dawley SD rats were randomly divided into three groups: stress group (n = 26), drug group (n = 12) and control group (n = 12). The rats in the stress group and the drug group were stimulated by chronic stress for 8 weeks. The drug group was given fluoxetine hydrochloride 10 mg / g / g I.P.E at the first week, and the sugar water preference test and open field experiment were performed after the 8th week. Rats in stress group were divided into stress sensitive group and stress adaptation group according to sugar water preference rate at the end of 8 weeks. After the stress, the rats in the four groups were killed and the levels of CREB and p-CREB in hippocampus and frontal lobe were detected by protein immunoimprint technique. Results at the end of 8 weeks, the sugar water preference rate of stress sensitive group was lower than that of stress adaptation group, drug group and control group (all P 0.05). The time of center lattice and the times of standing upright were lower than those of drug group and control group (P 0.05), but there was no significant difference between the control group and the stress adaptation group (all P 0.05). The levels of p-CREB in hippocampus and prefrontal lobe in stress sensitive group were significantly lower than those in stress adaptation group, drug group and control group (all P 0.05). Conclusion the level of CREB phosphorylation in hippocampus and frontal lobe may be related to the mechanism of emotional disorder induced by stress.
【作者单位】：上海交通大学医学院附属精神卫生中心心境障碍科;航天中心医院神经内科;复旦大学附属华山医院中西医结合研究所;
【基金】：上海交通大学科学基金(编号:11XJ21006) 上海市自然科学基金(编号:09ZR1427200) 上海市卫生局公共卫生海外人才项目(编号:GWHW201208) 国家高技术研究发展计划“863”计划(编号:2006AA02Z430) 国家自然科学基金重大计划项目(编号:91232719) 国家临床重点专科-上海市精神卫生中心(编号:卫生部医政司2011-873)
【分类号】：R749.4

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