IGF-1对PC12细胞PRNP表达及APP代谢的影响

发布时间：2018-06-10 02:57

本文选题：阿尔茨海默病 + 胰岛素样生长因子-　；参考：《上海交通大学学报(医学版)》2017年04期

【摘要】：目的·探讨胰岛素样生长因子-1(IGF-1)对肾上腺嗜铬细胞瘤细胞(PC12)中朊蛋白编码基因(PRNP)表达及淀粉样蛋白前体(APP)代谢的影响。方法·建立阿尔茨海默病(AD)细胞模型;用不同浓度的IGF-1(20、40、80 ng/mL)作用于PC12细胞24 h,采用real-time PCR检测PRNP m RNA的表达水平;Western blotting检测AKT/p AKT、ERK/p ERK蛋白的表达水平;ELISA检测细胞上清液中β-淀粉样蛋白42(Aβ42)的水平。结果·与空白对照组相比,AD模型组PRNP m RNA的表达量明显升高(P0.01);不同浓度IGF-1处理细胞24 h后,40 ng/mL及80 ng/mL IGF-1组PRNP m RNA的表达量显著升高(P0.01)。模型组及IGF-1各浓度组中APP蛋白的表达均无显著变化(P0.05)。与对照组相比,AD模型组上清液中Aβ42的水平显著下降,其他各组随着IGF-1浓度的增加而降低,其中40 ng/mL及80 ng/mL IGF-1组Aβ42的表达水平降低显著(P0.05)。与对照组相比,各组AKT/p AKT、ERK/p ERK蛋白的表达量均明显升高,且随着IGF-1剂量的增加而上升(P0.05)。结论·IGF-1降低PRNP基因表达的同时影响APP代谢,此过程可能与PI3K/AKT和MAPK/ERK1/2信号通路有关。
[Abstract]:Objective to investigate the effect of insulin-like growth factor-1 (IGF-1) on the expression of prion protein encoding gene (PRNPN) and metabolism of amyloid precursor (app) in adrenal pheochromocytoma cell line (PC12). Methods A model of Alzheimer's disease (AD) cells was established. PC12 cells were treated with different concentrations of IGF-1F-1 (4080ng / mL) for 24 h. The expression level of PRNP-mRNA in PC12 cells was detected by real-time PCR and the expression level of AKTP-pAK-ERKP / pERK protein was detected by Western blotting. The level of 尾 -amyloid protein 42A 尾 42 in the supernatant of PC12 cells was detected by Elisa. Results compared with the control group, the expression of PRNP mRNA in AD model group was significantly higher than that in control group, and the expression of PRNP mRNA in 40 ng / mL and 80 ng / mL IGF-1 group was significantly higher than that in control group after 24 h treatment with different concentrations of IGF-1. There was no significant change in the expression of app protein in the model group and IGF-1 concentration group. Compared with the control group, the level of A 尾 42 in the supernatant of AD model group decreased significantly, and the other groups decreased with the increase of IGF-1 concentration. The expression level of A 尾 42 in 40 ng / mL and 80 ng / mL IGF-1 group decreased significantly (P 0.05). Compared with the control group, the expression of AKT / p AKT / ERK / P ERK protein was significantly increased in each group, and increased with the increase of IGF-1 dose (P 0.05). Conclusion IGF-1 can decrease PRNP gene expression and affect app metabolism, which may be related to PI3K / AKT and MAPK / ERK1 / 2 signaling pathway.
【作者单位】：遵义医学院附属医院神经内科;
【基金】：贵州省科学技术基金项目(黔科合LH字[2015]7466号)~~
【分类号】：R749.16

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