精神分裂症患者血浆miRNA表达水平与精神症状的关系研究

发布时间：2018-06-12 12:53

本文选题：精神分裂症 + 微RNAs　；参考：《解放军医学杂志》2014年05期

【摘要】：目的探讨精神分裂症患者血浆中miRNA表达水平与精神症状的关系。方法连续纳入2012年7月-2013年5月收治的53例未经药物治疗的精神分裂症患者,采用实时荧光定量PCR技术检测血浆中9种miRNA(miR-30e、miR-34a、miR-181b、miR-195、miR-346、miR-432、miR-7、miR-132、miR-212)的表达水平,采用阳性和阴性症状量表(PANSS)评估其精神症状,并对结果进行统计学分析。结果 miR-34a与激活性症状及攻击性症状呈显著负相关(r= 0.345、 0.284,P0.05),miR-346与一般精神病理症状呈明显负相关(r= 0.282,P0.05)。miR-34a高表达组激活性症状群分和攻击性症状群分显著低于低表达组(P0.05),miR-346高表达组反应缺乏症状群分显著低于低表达组(P0.05)。miR-34a分别进入以激活性症状群和攻击性症状群为因变量的回归方程,可解释激活性症状变异的10.2%,攻击性症状群变异的6.3%;miR-346进入以一般精神病理症状为因变量的回归方程,可解释一般精神病理变异的6.1%。结论精神分裂症患者血浆中的miR-34a与激活性症状、攻击性症状有明显关联,miR-346与一般精神病理症状有明显关联,此二者可能在上述精神症状的发生发展过程中起重要作用。
[Abstract]:Objective to investigate the relationship between miRNA expression and psychiatric symptoms in patients with schizophrenia. Methods from July 2012 to May 2013, 53 patients with untreated schizophrenia were admitted to our hospital. The expression levels of nine miRNAs miR-30esimiR-34asimiR-181bmiR-195miR-346m miR-442 (miR-7miR-7miR-132miR-212) in plasma were detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). The positive and negative symptom scale (PANSS) was used to evaluate the psychiatric symptoms and the results were statistically analyzed. Results there was a significant negative correlation between miR-34a and active and aggressive symptoms (r = 0.345), and between 0.284 (P 0.05) and general psychopathological symptoms (r = 0.282) P0.05 + .miR-34a. The scores of active symptom group and aggressive symptom group in high expression group were significantly lower than those in low expression group (P0.05MR-346). The score of response deficiency group in high expression group was significantly lower than that in low expression group (P 0.05N. MiR-34a), which entered the regression equation with activated symptom group and aggressive symptom group as dependent variables, respectively. It can explain the variation of active symptom (10.2g) and aggressive symptom group (6.3miR-346) by entering the regression equation with general psychopathologic symptoms as dependent variable, and explaining the variation of general psychopathology by 6.1g. Conclusion miR-34a in plasma of schizophrenic patients is associated with active symptoms, aggressive symptoms and general psychopathological symptoms, which may play an important role in the occurrence and development of the above mental symptoms.
【作者单位】：江苏大学临床医学院;解放军102医院;第二军医大学心理与精神病学系;徐州医学院研究生院;GoPath
【基金】：南京军区2012年医学科技创新课题(12MA002)~~
【分类号】：R749.3

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