谷氨酸和皮质酮诱导的PC12抑郁症细胞模型差异性的~1H NMR代谢组学研究

发布时间：2018-06-13 19:44

本文选题：代谢组学 + 皮质酮　；参考：《药学学报》2017年02期

【摘要】：应用~1H NMR代谢组学技术分析皮质酮(corticosterone,CORT)和谷氨酸(glutamate,Glu)诱导PC12细胞后,细胞及其培养液中代谢产物的变化,比较诱导后的细胞与空白对照组代谢产物的差异性,并结合多元统计分析和代谢通路分析探讨两种诱导方式所致抑郁症细胞模型的特点,为抑郁症疾病研究中模型的选择及作用机制研究提供依据。结果显示在CORT刺激下,细胞内外共发现36个差异代谢产物;Glu刺激下细胞内外发现42个差异代谢产物。其中,两种方式刺激后细胞内外相同的差异代谢物分别有13个和19个。相关性分析结果显示CORT刺激下,丝氨酸和2-氧基异亮氨酸与多数差异代谢物相关;Glu刺激下,乳酸和谷胱甘肽与多数差异代谢物相关;由此推测,CORT刺激下,其影响的主要是脂肪和脂肪酸的转化以及细胞膜结构,而Glu刺激下,主要对糖酵解过程以及抗氧化过程有影响。
[Abstract]:The changes of metabolites in PC12 cells induced by corticosterone (Cort) and glutamate Glu) were analyzed by 1H NMR. Combined with multivariate statistical analysis and metabolic pathway analysis, the characteristics of the cell model of depression induced by two induction modes were discussed, which provided the basis for the selection of the model and the study of the mechanism of action in the study of depression disease. The results showed that 36 different metabolites and 42 different metabolites were found in and out of the cells stimulated by Cort. There were 13 and 19 different metabolites inside and outside the cells after stimulation in two ways. The results of correlation analysis showed that lactic acid and glutathione were related to most different metabolites under Cort stimulation, serine and 2-oxy isoleucine stimulated by most differential metabolites. The transformation of fat and fatty acids and the structure of cell membrane were mainly affected by the effects of Glu on the glycolysis process and the antioxidant process.
【作者单位】：山西大学中医药现代研究中心;山西大学体育学院;中国医学科学院北京协和医学院药物研究所;
【基金】：国家自然科学基金资助项目(81441096) 山西省科技计划资助项目(201603D321077) 山西省科技创新重点团队资助项目(201605D131045-18) 山西省重点实验室资助项目(201605D111004)
【分类号】：R749.4

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