BDNF基因G196A多态性与散发性阿尔茨海默病的相关性研究

发布时间：2018-06-18 23:52

本文选题：阿尔茨海默病 + 脑源性神经营养因子　；参考：《广西医科大学》2012年硕士论文

【摘要】：目的：探讨中国人群中脑源性神经营养因子（BDNF）基因G196A单核苷酸多态性与散发性阿尔茨海默病（AD）的关系。方法：采用聚合酶链反应一限制性片段长度多态性(PCR．RFLP)技术检测58例散发性AD患者和52例健康对照者多态性位点：BDNF G196A(Valiner-Meflfionine）（基因库编码rs6265）的单核苷酸多态性(SNP)在中国人群中的分布，分析各基因型与性别及疾病严重程度的关系。采用非条件logistic回归模型分析比较携带不同基因型人群罹患AD的风险。结果：1、AD组和对照组年龄(P=0.964)及性别(P=0.976)均无显著性差异，，AD及对照组BDNF基因G196A位点基因型及等位基因频率分布均符Hardy-Weinberg平衡定律AD组χ2=0.064，P=0.800；对照组χ2=0.076，P=0.783，两组基因型及等位基因频率分布均无显著性差异（分别为χ2=0.013，P=0.9940.05，χ2=0.013，P=0.9100.05)。进一步将AD组按照性别分层，也未发现差异有统计学意义(P0.05)。 2、BDNF基因G196A位点SNP与AD患病风险的关系：与GG基因型相比，携带AA基因型个体与AD的患病风险无统计学关联（OR＝0.942，95％CI：0.333～2.670），携带AG基因型个体与AD的患病风险无统计学关联（OR＝0.980，95％CI：0.399～2.404），携带A等位基因的基因型个体与AD的患病风险亦无统计学关联（OR＝1.034，95％CI：0.446～2.397）。 3、BDNF基因G196A位点的各基因型与AD的严重程度不存在相关性。结论:中国人群中BDNF基因G196A位点SNP的分布可能与AD无关联；中国人群中BDNF基因G196A位点SNP可能与AD患病风险无关联，均需扩大样本量及多中心研究证实。
[Abstract]:Objective: to investigate the relationship between G196A single nucleotide polymorphism (SNP) of brain-derived neurotrophic factor (BDNF) gene and sporadic Alzheimer's disease (ADD) in Chinese population. Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR.RFLP) technique was used to detect the single nucleotide polymorphism (SNPs) at the polymorphic site: BDNF G196A1 Valiner-Meflfionine (gene pool coding rs6265) in 58 sporadic AD patients and 52 healthy controls. The distribution of people in China, The relationship between genotype and sex and severity of disease was analyzed. Non-conditional logistic regression model was used to analyze the risk of AD in people with different genotypes. Results there was no significant difference between AD group and control group (P < 0.964) and sex (P < 0.976). The genotype and allele frequency distribution of G196A locus of BDNF gene in AD group and control group were all Hardy-Weinberg equilibrium law. There was no significant difference in the frequency distribution of genotype and allele between the two groups (蠂 2 / 0.013 P = 0.9940.05, 蠂 ~ 2 = 0.013 P = 0.9100.05, respectively). Further stratification according to sex in AD group, there was no significant difference between SNP at G196A locus of BDNF gene and the risk of AD: compared with GG genotype, there was no significant difference between SNP and GG genotype. There was no statistical association between the individuals with AA genotype and the risk of AD. There was no statistical correlation between the individuals with AA genotype and the risk of AD. There was no statistical association between individuals with genotype AG and the risk of AD. There was no significant correlation between individuals with genotype AG and the risk of AD. There was no significant correlation between individuals with AA genotypes and the risk of AD. There was no statistical association between individuals with AA genotypes and the risk of AD. There was also no significant correlation between the risk of AD and the risk of AD. There was no correlation between the genotype of the G196A locus of BDNF gene and the severity of AD. Conclusion: the distribution of SNP at the G196A locus of BDNF gene may not be associated with AD in Chinese population, but there may be no association between SNP at G196A locus of BDNF gene and the risk of AD in Chinese population.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.16

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