重复经颅磁刺激对阿尔茨海默病大鼠脑电LZ复杂度和脑白质中碱性髓鞘蛋白分布的影响

发布时间：2018-06-28 13:40

本文选题：阿尔茨海默病 + 重复经颅磁刺激　；参考：《实用医学杂志》2017年15期

【摘要】：目的探讨重复经颅磁刺激(r TMS)对阿尔茨海默病(AD)大鼠学习记忆能力影响的可能机制。方法挑选学习记忆无差异的SD大鼠90只,随机挑选30只作为对照组;大鼠双侧海马区注射Aβ1-42建立AD模型,用Morris水迷宫和免疫组化验证;将造模成功的大鼠随机分为AD组和(r TMS+AD)组,各组30只;(r TMS+AD)组施加r TMS,对照组和AD组施加伪刺激;干预完成后,进行水迷宫实验、脑电采集与分析、免疫组化实验以及统计分析。结果 (1)AD组较对照组大鼠逃避潜伏期变长,距离平台平均距离变远;(r TMS+AD)组较AD组大鼠逃避潜伏期变短,距离平台平均距离减小,差异有统计学意义(P0.05)。(2)AD组较对照组大鼠脑电LZ复杂度降低,(r TMS+AD)组较AD组大鼠脑电的LZ复杂度升高,差异有统计学意义(P0.05)。(3)对照组脑组织未见斑块状沉淀,细胞形态完好,轮廓清晰,排列整齐,髓鞘着色较深,分布长且连续;AD组脑组织中出现斑块状沉淀,细胞萎缩聚集,轮廓模糊,排列混乱,髓鞘染色较浅,分布短且不连续;(r TMS+AD)组较AD组脑组织中细胞萎缩情况减轻,髓鞘染色变深,分布增加。结论 r TMS可增加脑组织中髓鞘分布,提高AD大鼠脑电LZ复杂度,改善AD大鼠的学习记忆能力。
[Abstract]:Objective to investigate the possible mechanism of repetitive transcranial magnetic stimulation (r TMS) on learning and memory ability in Alzheimer's disease (AD) rats. Methods 90 Sprague-Dawley rats with no difference in learning and memory were selected and 30 rats were randomly selected as control group. The AD model was established by injecting A 尾 1-42 into bilateral hippocampal area of rats, and was verified by Morris water maze and immunohistochemistry. The rats were randomly divided into AD group (n = 30) and (r TMS AD) group (n = 30) and control group (n = 30) and AD group (n = 30). Immunohistochemical experiment and statistical analysis. Results (1) the escape latency of AD group was longer than that of control group, and the average distance from platform to; (r TMS AD group was shorter than that of AD group. The difference was statistically significant (P0.05). (2). Compared with the control group, the LZ complexity of the AD group was lower than that of the control group. The LZ complexity of the AD group was significantly higher than that of the AD group. The difference was statistically significant (P0.05). (3) there was no plaque deposition in the brain tissue of the AD group, and the morphology of the cells was intact. In AD group, there were plaques in brain tissue, cell atrophy and aggregation, vague outline, disordered arrangement and light staining of myelin sheath. In the short and discontinuous; (r TMS AD group, the cell atrophy in brain tissue was reduced, the staining of myelin sheath became deeper and the distribution increased than that in the AD group.
【作者单位】：河北省涿州市医院;新乡医学院;
【基金】：河南省生物精神病重点实验室开放课题(编号:ZDSYS2014005)
【分类号】：R749.16

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