白藜芦醇对阿尔茨海默病模型大鼠海马组织星形胶质细胞及TNF-α表达的影响

发布时间：2018-07-10 16:42

本文选题：白藜芦醇 + 阿尔茨海默病　；参考：《卫生研究》2015年04期

【摘要】：目的观察不同浓度白藜芦醇(Res)对阿尔茨海默病(AD)大鼠海马组织星形胶质细胞及TNF-α表达的影响。方法将60只Wistar雌性大鼠分为6组:假手术组,模型组,Res 20、40、80 mg/kg及戊酸雌二醇组(0.8 mg/kg),利用去卵巢合并D-半乳糖(100 mg/kg)腹腔注射建立AD模型,干预12周后,采用在体心脏灌流,固定海马组织,通过免疫组织化学法检测海马星形胶质细胞及肿瘤坏死因子α(TNF-α)表达的变化。结果模型组胶质纤维酸性蛋白(GFAP)及TNF-α表达量均明显高于假手术组(P0.01)。Res 20 mg/kg组GFAP表达量与模型组比较,差异无统计学意义;Res 40、80 mg/kg及戊酸雌二醇组GFAP表达量明显低于模型组(P0.01),而且随着Res浓度的增高,GFAP的表达量逐渐下降。Res 20、40、80 mg/kg,戊酸雌二醇组TNF-α表达量明显低于模型组(P0.01)。结论 Res可通过抑制星形胶质细胞增殖活化以及TNF-α的分泌,进而改善AD大鼠的脑内炎症反应。
[Abstract]:Aim to observe the effects of resveratrol (res) at different concentrations on the expression of astrocytes and TNF- 伪 in hippocampus of rats with Alzheimer's disease (AD). Methods Sixty female Wistar rats were divided into 6 groups: sham operation group, model group (Res 20 + 4080 mg/kg) and estradiol valerate group (0. 8 mg/kg). The AD model was established by intraperitoneal injection of ovariectomized with Dgalactose (100 mg/kg). The expression of astrocytes and tumor necrosis factor- 伪 (TNF- 伪) in hippocampal astrocytes were detected by immunohistochemical method. Results the expression of glial fibrillary acidic protein (GFAP) and TNF- 伪 in the model group was significantly higher than that in the sham-operated group (P0.01) .Res20 mg/kg group and the model group. The expression of GFAP in Res4080 mg/kg and Estradiol valerate groups was significantly lower than that in the model group (P0.01), and the expression of GFAP decreased gradually with the increase of res concentration. The expression of TNF- 伪 in Estradiol valerate group was significantly lower than that in the model group (P0.01). Conclusion res can improve the inflammatory response of AD rats by inhibiting the proliferation, activation of astrocytes and secretion of TNF- 伪.
【作者单位】：山西医科大学公共卫生学院营养与食品卫生学教研室;
【基金】：国家自然科学基金(No.81472984,81001245) 2013年度山西医科大学科技创新基金(No.01201301)
【分类号】：R749.16

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