山楂叶总黄酮对血管性痴呆大鼠海马谷氨酸和NMDA受体表达的影响

发布时间：2018-07-16 18:21

【摘要】：目的探讨山楂叶总黄酮对血管性痴呆大鼠海马谷氨酸含量及其NMDA受体表达的影响。方法雄性SD大鼠随机分为假手术组、模型组、尼莫地平组及山楂叶总黄酮Ⅰ、Ⅱ、Ⅲ组,每组12只,永久性结扎双侧颈总动脉法制备血管性痴呆大鼠模型,尼莫地平组及山楂叶总黄酮各组给予相应剂量药物灌胃,模型组及假手术组给予等量生理盐水,采用避暗实验观察大鼠学习记忆能力,电镜观察海马神经元超微结构,免疫组化法检测海马组织谷氨酸含量及其NMDA受体表达。结果与模型组比较,山楂叶总黄酮70、140、280 mg/kg剂量依赖性地增加大鼠逃避潜伏期(P0.05,P0.01),减少错误次数(P0.05,P0.01),提高选择正确率,显著改善大鼠学习记忆障碍;谷氨酸及其NMDA受体免疫阳性细胞积分光密度显著增加(P0.05,P0.01)。结论山楂叶总黄酮能显著改善大鼠学习记忆障碍,机制可能与其有效调节海马内谷氨酸、NMDA受体的表达有关。
[Abstract]:Objective to investigate the effect of total flavonoids of Hawthorn leaves on the content of glutamate and the expression of NMDA receptor in hippocampus of vascular dementia rats. Methods male Sprague-Dawley rats were randomly divided into sham operation group, model group, nimodipine group and Hawthorn total flavonoid group (12 rats in each group). Vascular dementia rats were made by permanent ligation of bilateral common carotid artery. Nimodipine group and Hawthorn leaf total flavonoids group were given the corresponding dose of drug by stomach, the model group and sham operation group were given the same amount of normal saline, the learning and memory ability of rats was observed by dark avoidance experiment, and the ultrastructure of hippocampal neurons was observed by electron microscope. The content of glutamate and the expression of NMDA receptor in hippocampus were detected by immunohistochemical method. Results compared with the model group, the total flavonoids of Hawthorn leaves in 70140280 mg/kg dose-dependently increased the escape latency (P0.05V, P0.01), decreased the number of errors (P0.05P0.01), increased the correct rate of selection, and significantly improved the learning and memory impairment in rats. The integral optical density of glutamate and its NMDA receptor immunoreactive cells increased significantly (P0.05, P0.01). Conclusion Total flavonoids in Hawthorn leaves can significantly improve learning and memory impairment in rats. The mechanism may be related to its effective regulation of the expression of NMDA receptor in hippocampus.
【作者单位】：承德医学院病原生物学教研室;承德医学院基础医学研究所;
【基金】：河北省医学科学研究重点项目(编号:20120160)
【分类号】：R749.13

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