丰富环境对拟老年性痴呆小鼠学习记忆和神经元突触可塑性影响研究

发布时间：2018-07-28 14:57

【摘要】：目的：本实验研究丰富环境对拟老年性痴呆(Alzheimer disease,AD)小鼠学习记忆能力的影响,并探讨其可能的作用机制,为丰富环境干预AD临床推广应用提供理论依据。方法：健康昆明种小鼠40只随机分为标准环境对照组、标准环境模型组、丰富环境模型组和丰富环境对照组,每组10只。采用侧脑室注射凝聚态Aβ1-40建立拟AD小鼠模型,Morris水迷宫实验测试学习记忆能力；透射电镜观察海马CA1区突触数密度(Nv)、突触面密度(Sv)及平均面积(S)的变化；用免疫组化和图象分析方法检测海马CA1区突触素表达。结果：1. Morris水迷宫实验结果：标准环境模型组与标准环境对照组比较平均逃避潜伏期延长(P0.05),跳台次数减少(P0.05)。丰富环境模型组与标准环境模型组比较平均逃避潜伏期时间缩短(P0.05),跳台次数增多(P0.05)。 2.突触形态结构可塑性检测显示：标准环境对照组较标准环境模型组突触数密度和面密度较大(P0.01),连接带平均面积也较小(P0.01)。丰富环境模型组较标准环境模型组突触数密度和面密度明显增大(P0.01),连接带平均面积也相对较小(P0.01)。 3.突触素免疫组化检测结果：标准环境对照组和丰富环境对照组突触素表达最多,丰富环境模型组次之、标准环境模型组表达最少。通过检测光密度值定量分析显示：标准环境模型组与标准环境对照组比较突触素的平均光密度值明显降低(P0.01)；与标准环境模型组比较,丰富环境模型组的光密度均值明显增高(P0.01)。结论：1.丰富环境能改善AD小鼠学习记忆能力。 2.丰富环境可减轻AD小鼠突触的损伤,并促进新突触与突触连接的形成,促进突触的重构。丰富环境能改善AD小鼠学习记忆能力可能与突触可塑性有关。 3.丰富环境可使AD小鼠海马区突触素的表达增多,促使突触的重构,从而改善AD小鼠学习记忆能力。
[Abstract]:Objective: to study the effect of rich environment on learning and memory ability of Alzheimer disease (AD) mice and explore its possible mechanism. Methods: forty healthy Kunming mice were randomly divided into three groups: the standard environment control group, the standard environment model group, the enriched environment model group and the rich environment control group, with 10 mice in each group. The rat model of AD was established by injecting condensed matter A 尾 1-40 into the lateral ventricle. The ability of learning and memory was tested by Morris water maze test, and the changes of synaptic surface density (Sv) and average area (S) (S) of synaptic number density (Nv),) in hippocampal CA1 region were observed by transmission electron microscope (TEM). Synaptophysin expression in hippocampal CA1 was detected by immunohistochemistry and image analysis. The result is 1: 1. Morris water maze test results: standard environmental model group compared with the standard environment control group average escape latency prolonged (P0.05), the number of platform jumping decreased (P0.05). Compared with the standard environmental model group, the average escape latency time of the rich environment model group was shorter (P0.05), and the frequency of platform jumping increased (P0.05). The synaptic morphological and structural plasticity test showed that the number density and surface density of synapses in the standard environment control group were higher than those in the standard environmental model group (P0.01), and the average area of the junction band was also smaller (P0.01). Compared with the standard environmental model group, the synaptic number density and surface density of the rich environment model group increased significantly (P0.01), and the average area of the junction band was also relatively small (P0.01). The expression of synaptophysin was the most in the standard environment control group and the rich environment control group, followed by the rich environment model group and the standard environment model group. Quantitative analysis of optical density showed that the average optical density of synaptophysin in the standard environment model group was significantly lower than that in the standard environment control group (P0.01), and that in the enriched environment model group was significantly higher than that in the standard environment model group (P0.01). Conclusion 1. Rich environment can improve the learning and memory ability of AD mice. 2. Enriched environment can reduce synaptic damage, promote the formation of new synapses and synaptic connections, and promote synaptic remodeling in AD mice. Rich environment can improve learning and memory ability of AD mice, which may be related to synaptic plasticity. 3. 3. Rich environment can increase the expression of synaptophysin in hippocampal area of AD mice, promote synaptic remodeling, and improve the learning and memory ability of AD mice.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.16

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