大学生睾酮水平对脑认知功能影响的事件相关电位研究

发布时间：2018-09-04 16:28

【摘要】：目的：利用事件相关电位技术，使用2-back范式，在不同的睾酮水平下对字母匹配任务和空间匹配任务进行测试，以观察工作记忆的行为学和脑电波记录的结果，分析不同的睾酮水平下不同工作记忆任务的差异，同时也反映出睾酮对脑功能的影响。方法：本研究从大连医科大学2011年445名新入学男性中随机抽取100名进行血清睾酮水平测验，100名男生被试均为正常健康人。然后根据100名被试者血清睾酮水平按四分位法分为四个组，然后将最高睾酮水平组25人和最低睾酮水平组25人为研究对象，在征询被试同意后，排除无内分泌生殖疾病史、无精神疾病史、无外伤史和无其他身体疾病史者，年龄限制在17-19岁，均为右利手，智力水平无显著差异。最后选出12人入组高血清睾酮水平组，12人入组低血清睾酮水平组。刺激由E-prime1.0软件呈现，实验分为两个任务。第一个任务刺激内容为字母，第二个任务为空间位置的判断。两种类型的刺激任务分别都有150个刺激序列(Trial)，其中目标刺激和非目标刺激等概率随机出现（各50%），在非目标刺激中还有15个干扰刺激出现（占10%）。完成一个任务大约需10分钟，中间休息3分钟，完成整个实验大约需要30分钟左右。行为学结果反应时、命中率、误报率和干扰刺激反应时使用独立样本t检验方法进行两组之间比较，对事件相关电位成分采用重复测量的方差分析，并进一步进行事后分析。结果：（1）行为学结果中，高低睾酮组之间的字母匹配任务刺激反应时、命中率、误报率和干扰刺激反应时结果差别均不具有统计学意义，而刺激反应时和命中率方面差异具有统计学倾向性。高低睾酮组之间的空间匹配任务刺激结果发现命中率在高睾酮组显著高于低睾酮组，而在反应时、误报率和干扰刺激反应时差别均不具有统计学意义。（2）波形图中，在字母匹配刺激任务下，血清高睾酮水平组所有的位点都没有发现左中右激活的差异；而低血清睾酮水平组左中右间的激活差异较明显，主要表现在额区、中央区和顶区（F4,C4,P4,PZ）。在空间匹配任务下，高血清睾酮水平组在中央额区激活的波幅明显大于左右侧，在中央顶区激活的波幅明显比左右侧顶区大；低血清睾酮水平组在右侧额区的激活比左侧额区F3和中央额区FZ都明显；中央顶区的激活较两侧要明显。在其他位置波幅的差异不明显。（3）空间和字母匹配任务在280-400ms之间诱发的平均脑电地形图中，空间匹配任务血清睾酮水平高低两组的差异比较明显，而字母匹配任务的差异不是很明显，在空间匹配任务中高血清睾酮水平组的激活更明显一些，而在字母匹配任务中低血清睾酮水平组的激活则要相对较强。另外在两个匹配任务中血清低睾酮水平组的激活区域向右侧半球倾斜，而这种倾向在字母匹配任务中体现的更为明显，而高血清睾酮水平组的激活区域则主要集中在中央区域，向两侧倾斜的趋势不很明显。在图中还可以看出激活最强的区域主要集中在顶区，而中央区和前额叶的激活比较弱。（4）对空间和字母匹配任务在280-400ms之间诱发的平均脑电峰值进行统计分析，在字母匹配任务中，脑叶的主效应显著，脑区和组别的主效应不显著，脑区和组别的交互效应显著其他比较结果不显著。在空间匹配任务中，，脑叶主效应显著，脑区和组别的主效应不显著，脑区和组别以及脑叶、脑区和组别的交互效应都很显著，其他统计结果不显著。结论： 1.字母匹配任务，低睾酮水平组呈现了右侧半球代偿性优势激活状态，而高睾酮水平组，左右同时加工，差异不显著； 2.空间匹配任务，高睾酮水平组揭示了更高的加工状态，特别是中央区和顶区。
[Abstract]:AIM: To observe the behavior of working memory and the results of electroencephalogram (EEG) recording, analyze the differences of different working memory tasks at different levels of testosterone, and also reflect the effects of testosterone on brain function. Influence of energy.
Methods: 100 male students were randomly selected from 445 newly enrolled males of Dalian Medical University in 2011 to test their serum testosterone levels, and 100 male students were all healthy subjects. After consulting the consent of the subjects, the subjects were excluded from the group with no history of endocrine and reproductive diseases, no history of mental illness, no history of trauma, and no history of other physical diseases. The age was limited to 17-19 years old. They were right-handed and had no significant difference in intelligence level. The experiment was divided into two tasks. The first task was to stimulate the content of letters, the second task was to judge the spatial position. Both types of stimulus tasks had 150 stimulus sequences (Trial), in which the probability of target stimulus and non-target stimulus appeared randomly (50% each), and there were 15 stimuli in non-target stimulus. It takes about 10 minutes to complete a task, 3 minutes to rest, and about 30 minutes to complete the whole experiment. Analysis and further analysis afterwards.
Result:
(1) There were no significant differences in hitting rate, false alarm rate and interference stimulus response time between high and low testosterone groups, but there were significant differences in stimulus response time and hit rate between high and low testosterone groups. The high testosterone group was significantly higher than the low testosterone group, but there was no significant difference in response time, false alarm rate and interference stimulus response time.
(2) In the waveform diagram, there was no difference in activation between left, middle and right in the high serum testosterone level group under the letter-matching stimulation task, but there was a significant difference in activation between left, middle and right in the low serum testosterone level group, mainly in the frontal, central and parietal regions (F4, C4, P4, PZ). The activation amplitude in the central frontal region was significantly larger than that in the left and right frontal regions, and in the central parietal region was significantly larger than that in the left and right parietal regions.
(3) In the Average Brain Electrical Activity Map (AEEG) evoked by the spatial and alphabetic matching tasks between 280 and 400ms, the difference of serum testosterone level between the two groups was obvious, but the difference of the alphabetic matching task was not obvious. The activation of the high serum testosterone level group was more obvious in the spatial matching task, but in the alphabetic matching task. The activation of low serum testosterone level group was stronger than that of low serum testosterone level group. In addition, the activation region of low serum testosterone level group was inclined to the right hemisphere in the two matching tasks, which was more obvious in the letter matching task, while the activation region of high serum testosterone level group was mainly concentrated in the central region and inclined to both sides. It's not obvious. It's also shown that the most active areas are in the parietal region, while the central and prefrontal regions are weaker.
(4) Average EEG spikes induced by spatial and letter matching tasks between 280 and 400ms were statistically analyzed. In the letter matching task, the main effect of cerebral lobe was significant, the main effect of brain regions and groups was not significant, and the interaction effect of brain regions and groups was not significant. The main effects of regions and groups were not significant, and the interaction effects of brain regions and groups and lobes, brain regions and groups were significant, but other statistical results were not significant.
Conclusion:
1. In alphabet matching task, the low testosterone level group showed compensatory dominant activation in the right hemisphere, while the high testosterone level group, left and right processing at the same time, the difference was not significant.
2. in the spatial matching task, the high testosterone group revealed higher processing status, especially in the central and top regions.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R749.24

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