利培酮对大鼠各脑区组织BDNF-TrkB信号通路的影响
发布时间：2018-09-04 17:56
【摘要】：目的探索非典型抗精神病药利培酮对大鼠脑区中脑源性神经生长因子(brain-derived neurotrophic factor,BDNF)及其受体酪氨酸激酶受体B(tyrosine kinase receptor,TrkB)、P75神经营养因子受体(P75neurotrophin receptor,P75NTR)的调控作用。方法将大鼠随机分为利培酮处理组(n=8)和对照组(n=8),利培酮处理组采用0.25mg/kg利培酮腹腔内注射,对照组采用等量安慰剂腹腔内注射,连续处理14d后处死大鼠。取大鼠左右前额叶皮质、左右颞叶皮质及海马,提取RNA并进行BDNF、TrκB和P75 NTR mRNA的实时荧光定量PCR分析。结果利培酮处理组大鼠的左右前额叶皮质、左右颞叶皮质及海马中BDNF及TrkB mRNA的表达水平较对照组增高(P0.05),而利培酮处理组P75 NTR mRNA的表达与对照组间差异无统计学意义(P0.05)。结论利培酮能够上调大鼠左右前额叶皮质、左右颞叶皮质及海马BDNF-TrkB信号通路,而不能改变上述脑区中P75 NTR mRNA的表达水平。
[Abstract]:Objective to investigate the regulatory effect of risperidone, an atypical antipsychotic drug, on brain-derived nerve growth factor (brain-derived neurotrophic factor,BDNF) and its receptor tyrosine kinase receptor (B (tyrosine kinase receptor,TrkB) P75 neurotrophic factor receptor (P75neurotrophin receptor,P75NTR) in rat brain. Methods Rats were randomly divided into two groups: risperidone treated group and control group. The rats in risperidone treated group were injected intraperitoneally with 0.25mg/kg risperidone and those in control group were injected intraperitoneally with the same amount of placebo. The rats were sacrificed after 14 days of continuous treatment. RNA was extracted from left and right prefrontal cortex, left and right temporal lobe cortex and hippocampus, and real-time quantitative PCR analysis of BDNF,Tr 魏 B and P75 NTR mRNA was performed. Results the expression of BDNF and TrkB mRNA in prefrontal cortex, left and right temporal cortex and hippocampus of rats treated with risperidone was higher than that of control group (P0.05), while the expression of P75 NTR mRNA in risperidone treated group was not significantly different from that in control group (P0.05). Conclusion Risperidone can up-regulate the BDNF-TrkB signal pathway in the left and right prefrontal cortex, left and right temporal cortex and hippocampus of rats, but can not change the expression of P75 NTR mRNA in these brain regions.
【作者单位】： 四川大学华西医院精神医学研究室心理卫生中心;
【基金】：国家自然科学基金(No.81071089,No.81201042) 2013年度高等学校博士学科点专项科研基金(新教师类)(No.20130181120032)资助
【分类号】：R749.3
[Abstract]:Objective to investigate the regulatory effect of risperidone, an atypical antipsychotic drug, on brain-derived nerve growth factor (brain-derived neurotrophic factor,BDNF) and its receptor tyrosine kinase receptor (B (tyrosine kinase receptor,TrkB) P75 neurotrophic factor receptor (P75neurotrophin receptor,P75NTR) in rat brain. Methods Rats were randomly divided into two groups: risperidone treated group and control group. The rats in risperidone treated group were injected intraperitoneally with 0.25mg/kg risperidone and those in control group were injected intraperitoneally with the same amount of placebo. The rats were sacrificed after 14 days of continuous treatment. RNA was extracted from left and right prefrontal cortex, left and right temporal lobe cortex and hippocampus, and real-time quantitative PCR analysis of BDNF,Tr 魏 B and P75 NTR mRNA was performed. Results the expression of BDNF and TrkB mRNA in prefrontal cortex, left and right temporal cortex and hippocampus of rats treated with risperidone was higher than that of control group (P0.05), while the expression of P75 NTR mRNA in risperidone treated group was not significantly different from that in control group (P0.05). Conclusion Risperidone can up-regulate the BDNF-TrkB signal pathway in the left and right prefrontal cortex, left and right temporal cortex and hippocampus of rats, but can not change the expression of P75 NTR mRNA in these brain regions.
【作者单位】： 四川大学华西医院精神医学研究室心理卫生中心;
【基金】：国家自然科学基金(No.81071089,No.81201042) 2013年度高等学校博士学科点专项科研基金(新教师类)(No.20130181120032)资助
【分类号】：R749.3
【共引文献】
相关期刊论文 前10条
1 陈甲;张涛;刘娟;蒋晓江;许志强;;proBDNF对培养鼠小脑颗粒细胞增殖和凋亡的影响[J];第三军医大学学报;2014年19期
2 潘信明;周宏生;张晓燕;毛红军;华富江;姚丽敏;胡晓军;甘德芳;;利培酮对精神分裂症首次发病患者血清脑源性神经营养因子水平的影响[J];临床精神医学杂志;2013年06期
3 陈甲;矫树生;梁春荣;王延江;许志强;;脑源性神经营养因子前体对小鼠海马神经元存活和突起生长的抑制作用[J];解放军医学杂志;2014年09期
4 朱虹;贾z严，
本文编号：2222914
本文链接：https://www.wllwen.com/yixuelunwen/jsb/2222914.html
最近更新
教材专著
