桦褐孔菌对血管性痴呆模型大鼠的保护作用

发布时间：2018-09-12 10:29

【摘要】：目的:探讨桦褐孔菌对血管性痴呆(VD)大鼠行为学及组织病理形态的影响,阐明桦褐孔菌对VD大鼠的保护作用。方法:选用Wistar大鼠100只,采用反复夹闭大鼠双侧颈总动脉再灌注联合腹腔注射硝普钠降压法制备VD模型,大鼠分为正常对照组,模型组,阳性药组(吡拉西坦,0.5g·kg-1),低、中、高剂量(0.5、1.0和2.0g·kg-1)桦褐孔菌组,连续灌胃给药8周后,观察大鼠行为学和组织病理学变化。结果:Morris水迷宫实验,与模型组比较,中和高剂量桦褐孔菌组大鼠在实验第2~6天,到达平台的潜伏期和游程明显缩短(P0.05或P0.01),实验第7天穿越平台次数、穿越有效区次数、有效区停留时间、有效区停留距离、有效区停留时间/总时间和有效区停留距离/总路程均明显增加(P0.05),高剂量桦褐孔菌组大鼠平台停留时间和平台停留时间/总时间明显增加(P0.05)。组织病理形态观察,与模型组比较,高剂量桦褐孔菌组大鼠脑组织皮质区和海马区的病理性损伤明显减轻。结论:桦褐孔菌对VD大鼠具有保护作用,能改善VD大鼠的学习记忆能力,减轻VD大鼠脑组织的病理损伤。
[Abstract]:Aim: to investigate the effects of Betula obliquus on the behavior and histopathology of vascular dementia (VD) rats, and to elucidate the protective effect of Betula obliquus on VD rats. Methods: 100 Wistar rats were divided into normal control group, model group and positive drug group (Piracetam 0.5 g kg-1) by repeated clamping of bilateral common carotid artery reperfusion and intraperitoneal injection of sodium nitroprusside to reduce blood pressure. The behavior and histopathological changes of rats in high dose (0.5 g kg-1 and 2.0 g kg-1) group were observed after 8 weeks of continuous intragastric administration. Results compared with the model group, the incubation period and the running time of the rats in the middle and high dose group of Betula obliquus on the 2nd day of the experiment were significantly shorter than those in the model group (P0.05 or P0.01). On the 7th day of the experiment, the number of times of crossing the platform and the number of times of crossing the effective area were significantly shortened (P0.05 or P0.01). The effective zone residence time, effective area residence time / total time and effective area residence distance / total distance were significantly increased (P0.05), and the platform residence time and platform residence time / total time of high dose Betula obliquus group were significantly increased (P0.05). Histopathologic observation showed that the pathological damage in cortex and hippocampus of rats in high dose group was significantly reduced compared with the model group. Conclusion: Betula obliquus has protective effect on VD rats, can improve the ability of learning and memory of VD rats, and alleviate the pathological damage of brain tissue of VD rats.
【作者单位】：吉林大学药学院药理学教研室;
【基金】：吉林省科技厅科技引导计划项目资助课题(201205020)
【分类号】：R749.13;R-332

【相似文献】