氧化应激对溶酶体储存与转运甲醛的影响
发布时间：2018-10-13 10:31
【摘要】：内源甲醛代谢失调被认为是导致阿尔茨海默病的危险因素之一,甲醛蓄积会引起神经细胞的死亡和认知功能的降低.研究表明,细胞内甲醛分布于溶酶体内,而溶酶体功能异常与神经退行性疾病密切相关.本文采用甲醛特异荧光探针,在氧化应激条件下,检测到小鼠脑微血管内皮细胞株bEnd.3和小鼠神经瘤母细胞株N2a溶酶体内甲醛明显升高;在慢性脑低灌注大鼠动物模型中,其脑神经细胞的溶酶体内甲醛也升高(P0.01);LeuLeuOMe处理bEnd.3细胞,使其溶酶体膜通透性增加,导致细胞内甲醛蓄积,而胞外甲醛降低.以上结果证明,溶酶体具有储存和转运甲醛的功能,如果溶酶体出现结构与功能的异常,会导致甲醛代谢失调,造成认知损害.
[Abstract]:Endogenous formaldehyde metabolism disorder is considered to be one of the risk factors leading to Alzheimer's disease. Formaldehyde accumulation can lead to the death of nerve cells and the decrease of cognitive function. The results showed that formaldehyde in cells was located in lysosomes, and abnormal lysosomal function was closely related to neurodegenerative diseases. Under oxidative stress, formaldehyde in lysosome of mouse brain microvascular endothelial cell line bEnd.3 and mouse neuroblastoma cell line N2a was detected by formaldehyde specific fluorescence probe. The level of formaldehyde in lysosome of brain nerve cells was also increased (P0.01); LeuLeuOMe treatment increased the permeability of lysosomal membrane of bEnd.3 cells, resulting in the accumulation of formaldehyde in cells and the decrease of extracellular formaldehyde. These results suggest that lysosomes can store and transport formaldehyde. If the lysosome has abnormal structure and function, it will lead to maladjustment of formaldehyde metabolism and cognitive impairment.
【作者单位】： 中国科学院生物物理研究所脑与认知科学国家重点实验室;中国科学院大学;
【基金】：国家重点基础研究发展计划(973)资助项目(2012CB911004)~~
【分类号】：R749.16
本文编号：2268295
[Abstract]:Endogenous formaldehyde metabolism disorder is considered to be one of the risk factors leading to Alzheimer's disease. Formaldehyde accumulation can lead to the death of nerve cells and the decrease of cognitive function. The results showed that formaldehyde in cells was located in lysosomes, and abnormal lysosomal function was closely related to neurodegenerative diseases. Under oxidative stress, formaldehyde in lysosome of mouse brain microvascular endothelial cell line bEnd.3 and mouse neuroblastoma cell line N2a was detected by formaldehyde specific fluorescence probe. The level of formaldehyde in lysosome of brain nerve cells was also increased (P0.01); LeuLeuOMe treatment increased the permeability of lysosomal membrane of bEnd.3 cells, resulting in the accumulation of formaldehyde in cells and the decrease of extracellular formaldehyde. These results suggest that lysosomes can store and transport formaldehyde. If the lysosome has abnormal structure and function, it will lead to maladjustment of formaldehyde metabolism and cognitive impairment.
【作者单位】： 中国科学院生物物理研究所脑与认知科学国家重点实验室;中国科学院大学;
【基金】：国家重点基础研究发展计划(973)资助项目(2012CB911004)~~
【分类号】：R749.16
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