阿尔茨海默病患者血小板脂质筏内神经节苷脂GM1含量的研究
发布时间：2018-10-24 11:30
【摘要】：目的研究阿尔茨海默病(AD)患者血小板脂质筏内神经节苷脂GM1含量的变化,寻求一种可能作为辅助诊断AD的早期生物学指标。方法选择42例AD患者和45例认知功能正常的健康对照者分别进行神经功能评定,采用以Optiprep为介质的密度梯度离心方法分离血小板脂质筏,应用点印迹的方法进行鉴定后,采用BCA法测定血小板脂质筏内蛋白含量,比色法及点印迹定量分析法测定其内神经节苷脂GM1含量。结果在离心管25%~30%Optiprep溶液界面处可见一条白色的闪亮环带,经点印迹法证实,位于第6层的此白色闪亮环带即为脂质筏。AD组血小板脂质筏内蛋白含量略高于对照组,但差异无统计学意义(P=0.1494)。AD组血小板脂质筏内神经节苷脂GM1含量明显高于对照组,差异有统计学意义(P0.01),但其与年龄、MMSE评分无相关性。结论 AD组患者血小板脂质筏内神经节苷脂GM1含量与对照组相比明显升高,故血小板脂质筏内神经节苷脂GM1含量的改变在AD的诊断中有一定的潜在价值,有望成为一种辅助诊断AD的早期生物学指标。
[Abstract]:Objective to study the changes of ganglioside GM1 in platelet lipid rafts in patients with Alzheimer's disease (AD) and to explore a possible early biological marker for AD diagnosis. Methods 42 patients with AD and 45 healthy controls with normal cognitive function were selected to evaluate their neurological function. Platelet lipid rafts were separated by density gradient centrifugation using Optiprep as the medium. The content of platelet lipid raft protein was determined by BCA method, and the content of ganglioside GM1 was measured by colorimetry and dot blot quantitative analysis. Results A white shiny band was observed at the interface of 25%~30%Optiprep solution in centrifuge tube. The white shiny band located in the sixth layer was identified as lipid raft by dot blotting. The protein content of platelet lipid raft in AD group was slightly higher than that in control group. But there was no significant difference (P < 0. 1494). AD). The content of ganglioside GM1 in platelet lipid raft was significantly higher than that in control group (P0. 01), but it had no correlation with age and MMSE score. Conclusion the content of ganglioside GM1 in platelet lipid raft in AD group is significantly higher than that in control group, so the change of GM1 content in platelet lipid raft may have some potential value in the diagnosis of AD. It is expected to be an early biological marker for auxiliary diagnosis of AD.
【作者单位】： 沈阳市第五人民医院神经内科;中国医科大学发育细胞生物学教研室;中国医科大学第一附属医院神经内科;
【基金】：沈阳市科技计划项目(F16-206-9-27)
【分类号】：R749.16
本文编号：2291274
[Abstract]:Objective to study the changes of ganglioside GM1 in platelet lipid rafts in patients with Alzheimer's disease (AD) and to explore a possible early biological marker for AD diagnosis. Methods 42 patients with AD and 45 healthy controls with normal cognitive function were selected to evaluate their neurological function. Platelet lipid rafts were separated by density gradient centrifugation using Optiprep as the medium. The content of platelet lipid raft protein was determined by BCA method, and the content of ganglioside GM1 was measured by colorimetry and dot blot quantitative analysis. Results A white shiny band was observed at the interface of 25%~30%Optiprep solution in centrifuge tube. The white shiny band located in the sixth layer was identified as lipid raft by dot blotting. The protein content of platelet lipid raft in AD group was slightly higher than that in control group. But there was no significant difference (P < 0. 1494). AD). The content of ganglioside GM1 in platelet lipid raft was significantly higher than that in control group (P0. 01), but it had no correlation with age and MMSE score. Conclusion the content of ganglioside GM1 in platelet lipid raft in AD group is significantly higher than that in control group, so the change of GM1 content in platelet lipid raft may have some potential value in the diagnosis of AD. It is expected to be an early biological marker for auxiliary diagnosis of AD.
【作者单位】： 沈阳市第五人民医院神经内科;中国医科大学发育细胞生物学教研室;中国医科大学第一附属医院神经内科;
【基金】：沈阳市科技计划项目(F16-206-9-27)
【分类号】：R749.16
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