GABRB2基因启动子区DNA甲基化与精神分裂症及其治疗的关系
发布时间：2018-10-24 19:46
【摘要】：目的探讨γ-氨基丁酸A型受体β2亚基(gamma-aminobutyric acid type A receptor beta2 subunit,GABRB2)基因启动子区DNA甲基化水平与精神分裂症及其药物治疗的关系。方法纳入精神分裂症患者53例和正常对照53名,采用Mass ARRY Epi TYPER DNA甲基化分析技术检测所有受试者外周血GABRB2基因启动子区DNA甲基化水平。患者组中12例在药物治疗8周后再次检测该基因启动子区DNA甲基化水平。结果患者组与对照组外周血GABRB2基因启动子区总甲基化和各Cp G位点的甲基化水平组间差异无统计学意义(P0.05)。经性别分层分析,患者组男性Cp G_28位点的甲基化水平较对照组男性降低[(0.215±0.084)vs.(0.264±0.103),P0.05]。治疗后患者组外周血GABRB2基因启动子区总DNA甲基化水平较治疗前降低[(0.088±0.037)vs.(0.121±0.063),P0.01],治疗前后各Cp G位点甲基化水平差异无统计学意义(P0.05)。结论抗精神病药物治疗可降低精神分裂症患者外周血中GABRB2基因启动子区DNA甲基化水平,提示基因启动子区DNA甲基化可能参与抗精神病药物治疗的作用机制。
[Abstract]:Objective to investigate the relationship between the level of DNA methylation in promoter region of 纬 -aminobutyric acid type A receptor 尾 2 subunit (gamma-aminobutyric acid type A receptor beta2 subunit,GABRB2) gene and schizophrenia and its drug therapy. Methods the DNA methylation levels in the promoter region of GABRB2 gene in peripheral blood of 53 patients with schizophrenia and 53 normal controls were detected by Mass ARRY Epi TYPER DNA methylation analysis. DNA methylation in the promoter region of the gene was detected again in 12 patients after 8 weeks of drug therapy. Results there was no significant difference in the total methylation of the promoter region of GABRB2 gene and the methylation level of each Cp G locus between the patients and the control group (P0.05). By sex stratification analysis, the methylation level of Cp G28 locus in the patient group was lower than that in the control group [(0.215 卤0.084) vs. (0.264 卤0.103), P0.05]. After treatment, the total DNA methylation level in the promoter region of GABRB2 gene in peripheral blood of the patients was lower than that before treatment [(0.088 卤0.037) vs. (0.121 卤0.063), P0.01]. There was no significant difference in the methylation level of each Cp G locus before and after treatment (P0.05). Conclusion antipsychotic therapy can reduce the level of DNA methylation in the promoter region of GABRB2 gene in the peripheral blood of patients with schizophrenia, suggesting that DNA methylation in the promoter region may be involved in the mechanism of antipsychotic drug therapy.
【作者单位】： 上海交通大学医学院附属精神卫生中心遗传室;
【基金】：国家自然科学基金(编号:81071094) 十二五科技重大专项“精神药物新药临床评价研究技术平台”(编号:2012ZX09303-003)
【分类号】：R749.3
，
本文编号：2292379
[Abstract]:Objective to investigate the relationship between the level of DNA methylation in promoter region of 纬 -aminobutyric acid type A receptor 尾 2 subunit (gamma-aminobutyric acid type A receptor beta2 subunit,GABRB2) gene and schizophrenia and its drug therapy. Methods the DNA methylation levels in the promoter region of GABRB2 gene in peripheral blood of 53 patients with schizophrenia and 53 normal controls were detected by Mass ARRY Epi TYPER DNA methylation analysis. DNA methylation in the promoter region of the gene was detected again in 12 patients after 8 weeks of drug therapy. Results there was no significant difference in the total methylation of the promoter region of GABRB2 gene and the methylation level of each Cp G locus between the patients and the control group (P0.05). By sex stratification analysis, the methylation level of Cp G28 locus in the patient group was lower than that in the control group [(0.215 卤0.084) vs. (0.264 卤0.103), P0.05]. After treatment, the total DNA methylation level in the promoter region of GABRB2 gene in peripheral blood of the patients was lower than that before treatment [(0.088 卤0.037) vs. (0.121 卤0.063), P0.01]. There was no significant difference in the methylation level of each Cp G locus before and after treatment (P0.05). Conclusion antipsychotic therapy can reduce the level of DNA methylation in the promoter region of GABRB2 gene in the peripheral blood of patients with schizophrenia, suggesting that DNA methylation in the promoter region may be involved in the mechanism of antipsychotic drug therapy.
【作者单位】： 上海交通大学医学院附属精神卫生中心遗传室;
【基金】：国家自然科学基金(编号:81071094) 十二五科技重大专项“精神药物新药临床评价研究技术平台”(编号:2012ZX09303-003)
【分类号】：R749.3
，
本文编号：2292379
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