Aβ1-42寡聚体对AD大鼠大脑皮质Bcl-2、Caspase-3表达的影响
发布时间：2018-11-06 11:16
【摘要】：目的探讨Aβ1-42寡聚体侧脑室灌注对大鼠大脑皮质Bcl-2、Caspase-3表达的影响。方法采用Morris水迷宫法筛选出逃避潜伏期少于60 s的60只雄性大鼠随机均分为4组:正常组、PBS对照组、Aβ1-42纤维组、Aβ1-42寡聚体组。右侧脑室注射Aβ1-42纤维体或Aβ1-42寡聚体复制阿尔茨海默病(AD)模型;PBS对照组注射等量的PBS;正常组不作任何处理。模型建立后第4周,采用Morris水迷宫测试大鼠学习记忆能力的变化,用RT-PCR法检测大鼠皮质Bcl-2、Caspase-3 mRNA的表达,用Western blot法检测AD大鼠皮质Bcl-2蛋白表达以及Caspase-3活性。结果造模后,与正常组和PBS对照组相比,Aβ1-42纤维组、Aβ1-42寡聚体组逃避潜伏期较造模前均延长(P0.05),Aβ1-42寡聚体组大鼠逃避潜伏期长于Aβ1-42纤维组大鼠的逃避潜伏期;Aβ1-42纤维组及Aβ1-42寡聚体组脑组织中Bcl-2表达均下调(P0.05),Caspase-3表达均上调(P0.05),以Aβ1-42寡聚体组较为显著。结论 Aβ1-42纤维、Aβ1-42寡聚体均可导致大鼠认知功能障碍,抑制大鼠皮质Bcl-2 mRNA表达,上调Caspase-3 mRNA表达,活化Caspase-3,其中,Aβ1-42寡聚体对Bcl-2 mRNA和Caspase-3 mRNA表达影响较大。
[Abstract]:Objective to investigate the effect of A 尾 1-42 oligomeric lateral ventricular perfusion on the expression of Bcl-2,Caspase-3 in rat cerebral cortex. Methods 60 male rats with escape latency less than 60 s were selected by Morris water maze method. They were randomly divided into 4 groups: normal group, PBS control group, A 尾 1-42 fiber group and A 尾 1-42 oligomer group. The (AD) model of Alzheimer's disease was induced by right ventricle injection of A 尾 1-42 fibrous body or A 尾 1-42 oligomer, while the PBS control group was injected with the same amount of PBS; without any treatment. At the 4th week after the establishment of the model, Morris water maze was used to test the changes of learning and memory ability in rats, and the expression of Bcl-2,Caspase-3 mRNA in cortex of rats was detected by RT-PCR method. The expression of Bcl-2 protein and the activity of Caspase-3 in cortex of AD rats were detected by Western blot method. Results compared with the normal group and PBS control group, the escape latency of A 尾 1-42 oligomer group was significantly longer than that of the control group (P0.05). The escape latency of A 尾 1-42 oligomer group was longer than that of A 尾 1-42 fiber group. In A 尾 1-42 fiber group and A 尾 1-42 oligomer group, the expression of Bcl-2 was down-regulated (P0.05) and the expression of Caspase-3 was up-regulated (P0.05), especially in A 尾 1-42 oligomer group. Conclusion A 尾 1-42 fibers and A 尾 1-42 oligomers can induce cognitive dysfunction, inhibit the expression of Bcl-2 mRNA in rat cortex, up-regulate the expression of Caspase-3 mRNA, and activate Caspase-3,. The expression of Bcl-2 mRNA and Caspase-3 mRNA was significantly affected by A 尾 1-42 oligodeoxynucleotides.
【作者单位】： 桂林医学院解剖学教研室;河北医科大学神经生物研究室;
【基金】：国家自然科学基金(编号:81260174) 2012年广西研究生创新项目(编号:YCSW2012108)
【分类号】：R749.16
[Abstract]:Objective to investigate the effect of A 尾 1-42 oligomeric lateral ventricular perfusion on the expression of Bcl-2,Caspase-3 in rat cerebral cortex. Methods 60 male rats with escape latency less than 60 s were selected by Morris water maze method. They were randomly divided into 4 groups: normal group, PBS control group, A 尾 1-42 fiber group and A 尾 1-42 oligomer group. The (AD) model of Alzheimer's disease was induced by right ventricle injection of A 尾 1-42 fibrous body or A 尾 1-42 oligomer, while the PBS control group was injected with the same amount of PBS; without any treatment. At the 4th week after the establishment of the model, Morris water maze was used to test the changes of learning and memory ability in rats, and the expression of Bcl-2,Caspase-3 mRNA in cortex of rats was detected by RT-PCR method. The expression of Bcl-2 protein and the activity of Caspase-3 in cortex of AD rats were detected by Western blot method. Results compared with the normal group and PBS control group, the escape latency of A 尾 1-42 oligomer group was significantly longer than that of the control group (P0.05). The escape latency of A 尾 1-42 oligomer group was longer than that of A 尾 1-42 fiber group. In A 尾 1-42 fiber group and A 尾 1-42 oligomer group, the expression of Bcl-2 was down-regulated (P0.05) and the expression of Caspase-3 was up-regulated (P0.05), especially in A 尾 1-42 oligomer group. Conclusion A 尾 1-42 fibers and A 尾 1-42 oligomers can induce cognitive dysfunction, inhibit the expression of Bcl-2 mRNA in rat cortex, up-regulate the expression of Caspase-3 mRNA, and activate Caspase-3,. The expression of Bcl-2 mRNA and Caspase-3 mRNA was significantly affected by A 尾 1-42 oligodeoxynucleotides.
【作者单位】： 桂林医学院解剖学教研室;河北医科大学神经生物研究室;
【基金】：国家自然科学基金(编号:81260174) 2012年广西研究生创新项目(编号:YCSW2012108)
【分类号】：R749.16
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