AD模型小鼠海马和小脑Aβ的沉积与相关miRNAs表达的变化

发布时间：2018-11-17 11:07

【摘要】：目的通过对比观察AD模型小鼠海马与小脑Aβ沉积和相关miRNAs表达的变化,探讨AD模型小鼠小脑是否存在Aβ沉积和相关miRNAs的差异表达。方法选择12月龄大小的APPswe/PS△E9双转基因AD小鼠作为实验组(EG),同月龄的同种系的野生型小鼠C57为对照组(CG),每组各12只。将小鼠脑组织分为左右两部分,右侧脑组织行刚果红染色检测淀粉样物质在海马及小脑的沉积;左侧脑组织分别用于提取海马及小脑的miRNA,应用实时荧光定量PCR方法分别检测两组小鼠海马及小脑组织的miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达情况。结果刚果红染色:实验组小鼠海马及小脑均可见橘红色的Aβ沉积,对照组小鼠海马及小脑均未见橘红色的Aβ沉积。实时荧光定量PCR:四种miRNAs在实验组海马的表达均低于对照组(P0.05);miRNA-135a-5p、miRNA-298-5p和miR-669f-3p在实验组小脑的表达低于对照组(P0.05);miRNA-298-5p和miR-669f-3p在实验组海马的表达低于小脑(P0.05)。结论 APPswe/PS△E9双转基因AD小鼠小脑中也存在Aβ沉积,其形成可能与miRNA-135a-5p、miRNA-298-5p和miR-669f-3p的表达下调有关。
[Abstract]:Objective to observe the changes of A 尾 deposition and related miRNAs expression in hippocampus and cerebellum of AD model mice, and to explore whether there is a difference between A 尾 deposition and related miRNAs expression in cerebellum of AD model mice. Methods 12 months old APPswe/PS E9 double transgenic AD mice were selected as experimental group (EG), homologous C57 mice of the same month age as control group, 12 mice in each group were selected as control group. The brain tissue of mice was divided into two parts: the right brain was stained with Congo red to detect the deposition of amyloid in hippocampus and cerebellum. The expression of miRNA-135a-5p,miRNA-298-5p,miRNA-466b-3p and miR-669f-3p in hippocampus and cerebellum of the two groups were detected by real-time fluorescence quantitative PCR method. Results Congo red staining: orange A 尾 was found in hippocampus and cerebellum of mice in experimental group, but not in hippocampus and cerebellum in control group. The expression of real-time quantitative PCR: miRNAs in hippocampus of experimental group was lower than that of control group (P0.05), and the expression of miRNA-135a-5p,miRNA-298-5p and miR-669f-3p in cerebellum of experimental group was lower than that of control group (P0.05). The expression of miRNA-298-5p and miR-669f-3p in hippocampus of experimental group was lower than that of cerebellum (P0.05). Conclusion there is also A 尾 deposition in cerebellum of APPswe/PS E9 double transgenic AD mice, which may be related to the down-regulation of miRNA-135a-5p,miRNA-298-5p and miR-669f-3p expression.
【作者单位】：中南大学湘雅三医院神经内科;
【基金】：湖南省科技厅社会发展支撑计划课题(2012SK3218)
【分类号】：R749.16

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