青春期认知功能干预对成年期甲基苯丙胺觅药行为的影响
发布时间：2018-11-21 17:02
【摘要】：目的 以甲基苯丙胺为代表的新型毒品，其滥用率和依赖性日渐严重。本实验采用自身给药模型与认知功能药物干预的方法，探求青春期不同时期认知功能干预对成年期甲基苯丙胺自身给药以及觅药行为的影响。 方法 实验用SD（Sprague-Dawley）大鼠。一半动物在青春期20天丰富、匮乏环境饲养后，进行20天的药物干预，另一半在青春期30天丰富、匮乏环境饲养后，进行20天的药物干预。其中，A和E组：20和30天丰富环境后，注射MK-801，连续20天。B和F组，20和30天丰富环境后，注射生理盐水，连续20天，作为A和E组的对照。C和G组：20和30天匮乏环境后，注射利培酮，连续20天。D和H组：20和30天匮乏环境后，注射生理盐水，连续20天，作为C和G组的对照。药物干预结束，进行水迷宫测试和甲基苯丙胺的自身给药实验。甲基苯丙胺自身给药训练剂量为0.03mg/kg/infusion或0.06mg/kg/infusion，每天4小时。随后进行FR（固定频率）、PR（渐变频率）剂量效应测试，所用剂量为0.015，0.03，，0.06，0.09和0.12mg/kg/infusion。FR测试每天4小时,PR测试每天6小时。戒断14天后,实验大鼠进行消退及重建测试。 结果 1、青春期第20天开始认知功能干预实验中，水迷宫测试A组动物与控制组B组相比潜伏期显著增加，C组动物与控制组D组相比显著降低；FR测试，在甲基苯丙胺剂量为0.015mg/kg/infusion时，A组动物有效鼻触数和给药次数相比于控制组B组显著增加，C组相比于控制组D组显著降低，并且，在甲基苯丙胺剂量为0.03mg/kg/infusion时，C组动物有效鼻触数和给药次数相比于控制组D组依然显著性降低，但是，在高剂量甲基苯丙胺0.06，0.09和0.12mg/kg/infusion时，各组之间有效鼻触数和给药针数无统计学差异；PR测试，在0.03mg/kg/infusion剂量A组动物甲基苯丙胺的断点数相比于控制组B组显著增加，而C组相比于控制组D组显著降低，其余四个剂量甲基苯丙胺，实验动物各组之间断点数无统计学差异；重建测试中，A组动物甲基苯丙胺重建率与控制组B组相比无统计学差异，而C组动物重建率与控制组D组相比显著降低。 2、青春期第30天开始认知功能干预实验中，水迷宫测试，E组动物潜伏期相比于控制组F组无显著性差异，G组相比于控制组H组显著降低；FR测试，五个甲基苯丙胺剂量下E组动物有效鼻触数和给药次数相比于控制组F组均无显著性差异，而G组动物在0.015mg/kg/infusion和0.03mg/kg/infusion剂量甲基苯丙胺的有效鼻触数和给药次数相比于控制组H组显著降低；PR测试，与FR测试相似的是，五个甲基苯丙胺剂量下E组动物甲基苯丙胺断点数相比于控制组F组无显著性差异，而G组动物在0.03mg/kg/infusion剂量下甲基苯丙胺断点数相比于控制组H组显著降低；重建测试，E组动物甲基苯丙胺重建率与F组相比无显著性差异，而G组动物重建率与控制组H组相比显著降低。 结论：匮乏组，无论青春期第20天还是30天开始认知功能干预，均对大鼠空间记忆、自身给药行为有影响。丰富组，只有青春期第20天开始药物干预才对大鼠空间记忆、自身给药行为有影响。而这种差异性主要是由药物干预的时间点不同造成的。认知功能增强剂可以逆转匮乏环境对大鼠空间记忆、给药动机、重建率的影响，并且无论青春期第20天还是第30开始干预，这种影响均显著；认知功能损害剂破坏了丰富环境对大鼠空间记忆、给药动机的保护作用，并且越早进行青春期认知功能药物干预，这种影响越明显。
[Abstract]:Purpose New drugs, represented by methamphetamine, with a growing abuse and dependence The effect of cognitive function in the different period of adolescence on the self-administration of methamphetamine and the behavior of drug administration was studied by means of self-administration model and cognitive function drug intervention. influence. Methods SD (Sprague-D, Wley rats. Half of the animals received 20 days of drug intervention after 20 days of puberty, and the other half in the 30-day period of puberty, and the other half took place after 30 days of puberty and in a deficient environment 20-day drug intervention, in which groups A and E: after 20 and 30 days of rich environment, MK-8 was injected 01, 20 days in succession. After 20 and 30 days of environment-rich environment, the saline was injected for 20 consecutive days. Control for Groups A and E. Group C and G: after 20 and 30 days of starvation, injection Capecone, continuous 20 days. Group D and H: after 20 and 30 days of starvation, saline was injected and continued for 20 days. Control for Group C and G. End of drug intervention, water maze test and methylstyrene-acrylic The self-administration of the amine was conducted with a dose of 0. 03mg/ kg/ infl or 0. 06mg/ kg/ infusi. On, 4 hours a day. The FR (fixed frequency), PR (gradient frequency) dose effect test was then performed, and the dose used was 0.015, 0.03, 0.06, 0.09 and 0.12mg/ kg/ infsion. The FR test was 4 hours a day, P R test 6 hours a day. After 14 days of withdrawal, the rats in the experiment line elimination Results 1. The latency of group B in water maze test group was significantly increased in group B compared with control group B, and group C animals were significantly lower than that of control group D. At the time of g/ kg/ infu, the effective nasal and administration times of group A animals were significantly increased compared with control group B, and group C was significantly lower in group C than in control group D, and when the dose of methamphetamine was 0. 03mg/ kg/ infsion, the effective nasal contact number and number of administration of group C animals were compared to control group In group D, there was a significant decrease in group D, but there was no significant difference in the number of effective nasal contact and the number of dosing needles between the groups at high-dose methamphetamine 0. 06, 0. 09 and 0. 12 mg/ kg/ infu. The PR test showed a significant increase in the number of broken points of methamphetamine in the group A of 0. 03mg/ kg/ infu, compared with the control group B group. There was no statistical difference between group C and group B in group C compared with group D, and there was no statistical difference between group B and group B in group A. the construction rate was significantly lower than that of the control group (D). In the first 30 days of puberty, there was no significant difference between the group F and the control group, and the G group was not significantly different from the control group F group. No significant difference was observed in group H compared to control group H; FR test, effective nasal contact number and number of administration of E-group animals in group E compared to control group F group, while group G animals had no significant difference in the number of effective nasal and administration of methamphetamine in 0. 015mg/ kg/ infl and 0. 03mg/ kg/ infu The PR test was similar to the control group H group; similar to the FR test, there was no significant difference in the number of methamphetamine break points in the E group at the five methylamphetamine doses compared to the control group F group, whereas the number of methamphetamine break points in the group G animals at the dose of 0. 03mg/ kg/ infu Compared with the control group, the group H significantly decreased; the reconstruction test showed no significant difference in the recovery rate of the methamphetamine in the E group compared with the group F, and the G group The animal reconstruction rate was significantly lower in comparison with the control group H group. Conclusion: The deficiency group, whether the 20-or 30-day period of puberty, started to cognize the function. Pre-treatment had an effect on the rat's spatial memory and the behavior of its own administration. The effect of the intervention on the rat's spatial memory and its self-administration behavior. The difference is mainly caused by the time point of the drug intervention. The cognitive function enhancer can reverse the effect of the deficient environment on the space memory, the administration motivation and the reconstruction rate of the rat. It is known that the functional damage agent has destroyed the protective effect of the rich environment on the space memory and the administration motivation of the rat, and the earlier it entered
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.64
本文编号：2347621
[Abstract]:Purpose New drugs, represented by methamphetamine, with a growing abuse and dependence The effect of cognitive function in the different period of adolescence on the self-administration of methamphetamine and the behavior of drug administration was studied by means of self-administration model and cognitive function drug intervention. influence. Methods SD (Sprague-D, Wley rats. Half of the animals received 20 days of drug intervention after 20 days of puberty, and the other half in the 30-day period of puberty, and the other half took place after 30 days of puberty and in a deficient environment 20-day drug intervention, in which groups A and E: after 20 and 30 days of rich environment, MK-8 was injected 01, 20 days in succession. After 20 and 30 days of environment-rich environment, the saline was injected for 20 consecutive days. Control for Groups A and E. Group C and G: after 20 and 30 days of starvation, injection Capecone, continuous 20 days. Group D and H: after 20 and 30 days of starvation, saline was injected and continued for 20 days. Control for Group C and G. End of drug intervention, water maze test and methylstyrene-acrylic The self-administration of the amine was conducted with a dose of 0. 03mg/ kg/ infl or 0. 06mg/ kg/ infusi. On, 4 hours a day. The FR (fixed frequency), PR (gradient frequency) dose effect test was then performed, and the dose used was 0.015, 0.03, 0.06, 0.09 and 0.12mg/ kg/ infsion. The FR test was 4 hours a day, P R test 6 hours a day. After 14 days of withdrawal, the rats in the experiment line elimination Results 1. The latency of group B in water maze test group was significantly increased in group B compared with control group B, and group C animals were significantly lower than that of control group D. At the time of g/ kg/ infu, the effective nasal and administration times of group A animals were significantly increased compared with control group B, and group C was significantly lower in group C than in control group D, and when the dose of methamphetamine was 0. 03mg/ kg/ infsion, the effective nasal contact number and number of administration of group C animals were compared to control group In group D, there was a significant decrease in group D, but there was no significant difference in the number of effective nasal contact and the number of dosing needles between the groups at high-dose methamphetamine 0. 06, 0. 09 and 0. 12 mg/ kg/ infu. The PR test showed a significant increase in the number of broken points of methamphetamine in the group A of 0. 03mg/ kg/ infu, compared with the control group B group. There was no statistical difference between group C and group B in group C compared with group D, and there was no statistical difference between group B and group B in group A. the construction rate was significantly lower than that of the control group (D). In the first 30 days of puberty, there was no significant difference between the group F and the control group, and the G group was not significantly different from the control group F group. No significant difference was observed in group H compared to control group H; FR test, effective nasal contact number and number of administration of E-group animals in group E compared to control group F group, while group G animals had no significant difference in the number of effective nasal and administration of methamphetamine in 0. 015mg/ kg/ infl and 0. 03mg/ kg/ infu The PR test was similar to the control group H group; similar to the FR test, there was no significant difference in the number of methamphetamine break points in the E group at the five methylamphetamine doses compared to the control group F group, whereas the number of methamphetamine break points in the group G animals at the dose of 0. 03mg/ kg/ infu Compared with the control group, the group H significantly decreased; the reconstruction test showed no significant difference in the recovery rate of the methamphetamine in the E group compared with the group F, and the G group The animal reconstruction rate was significantly lower in comparison with the control group H group. Conclusion: The deficiency group, whether the 20-or 30-day period of puberty, started to cognize the function. Pre-treatment had an effect on the rat's spatial memory and the behavior of its own administration. The effect of the intervention on the rat's spatial memory and its self-administration behavior. The difference is mainly caused by the time point of the drug intervention. The cognitive function enhancer can reverse the effect of the deficient environment on the space memory, the administration motivation and the reconstruction rate of the rat. It is known that the functional damage agent has destroyed the protective effect of the rich environment on the space memory and the administration motivation of the rat, and the earlier it entered
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.64
【参考文献】
相关期刊论文 前6条
1 张富强,周文华,杨国栋;觅药行为的神经生物学机制[J];国外医学.精神病学分册;1998年04期
2 夏国美;杨秀石;李骏;缪佳;;新型毒品滥用的成因与后果[J];社会科学;2009年03期
3 周吉银;莫志贤;;谷氨酸和受体与药物依赖相关的研究进展[J];中国药物依赖性杂志;2006年04期
4 马宝苗;张栗;吕秀依;刘昱;;大鼠静脉自身给药颈静脉插管手术方法的改进[J];中国药物依赖性杂志;2010年06期
5 吕秀依;翟海峰;马宝苗;刘昱;;药物静脉自身给药发展和药物强化作用认同的历史沿革[J];中国药物依赖性杂志;2011年01期
6 孙艳;马宝苗;黄坤玉;黄娴妮;杨澍均;刘昱;;大鼠甲基苯丙胺自身给药行为模型构建[J];中国药物依赖性杂志;2012年06期
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