硫化氢对创伤后应激障碍大鼠空间学习记忆能力及海马神经元放电的影响

发布时间：2018-12-07 10:37

【摘要】：目的探讨创伤后应激障碍(posttraumatic stress disorder,PTSD)大鼠行为学改变及脑内胱硫醚-β-合成酶(cysta-thionine-β-synthase,CBS)/硫化氢(hydrogen sulfide,H_2S)水平的变化,并研究外源性补充H_2S对大鼠行为学影响的作用及机制。方法应用连续单一应激方案制备创伤后应激障碍动物模型,用Morris水迷宫检测大鼠空间学习记忆能力,Western blot技术和亚甲基蓝法检测海马脑区CBS/H_2S体系含量,用多管微电极在体细胞外电生理记录海马神经元放电情况。结果 (1)与正常对照组比较,PTSD组大鼠逃避潜伏期增加(P0.01),在平台所在象限停留时间百分比和通过原平台位置次数明显减少(P0.01);海马脑区的CBS/H_2S体系含量明显降低(P0.01,P0.05)。(2)与PTSD组比较,PTSD+硫氢化钠(sodium hydrosulfide,NaH S)组大鼠逃避潜伏期减小(P0.01),在平台所在象限停留时间百分比和通过原平台位置次数明显增加(P0.01)。(3)微量压力注射L-半胱氨酸(L-cysteine)使海马神经元自发放电频率明显升高(P㩳0.01)。结论PTSD大鼠通过抑制海马脑区CBS/H_2S体系导致空间学习记忆能力降低。H_2S改善PTSD大鼠空间学习记忆能力的机制可能与其增加海马神经元放电频率和兴奋性有关。海马脑区CBS/H_2S体系导致空间学习记忆能力降低。H_2S改善PTSD大鼠空间学习记忆能力的机制可能与其增加海马神经元放电频率和兴奋性有关。
[Abstract]:Objective to investigate the behavioral changes and the changes of cysta-thionine- 尾 -synthase (cysta-thionine- 尾-synthase,CBS) / hydrogen sulfide (hydrogen sulfide,H_2S) levels in the brain of rats with post-traumatic stress disorder (posttraumatic stress disorder,PTSD). The effect and mechanism of exogenous H 2S supplementation on behavior of rats were studied. Methods Morris water maze was used to detect the spatial learning and memory ability of rats, and methylene blue method was used to detect the content of CBS/H_2S system in hippocampus. The discharge of hippocampal neurons was recorded by multitube microelectrode in external somatic electrophysiology. Results (1) compared with the normal control group, the escape latency of PTSD rats increased (P0.01), the percentage of staying time in the quadrant of the platform and the number of times passing through the original platform decreased significantly (P0.01). Compared with PTSD group, the content of CBS/H_2S system in hippocampus decreased significantly (P0.01). (2), and the escape latency of PTSD group (sodium hydrosulfide,NaH S) decreased (P0.01). The percentage of residence time in the quadrant of the platform and the number of times through the original platform position increased significantly (P0.01). (3). Microinjection of L-cysteine (L-cysteine) significantly increased the spontaneous discharge frequency of hippocampal neurons. (P0. 01). Conclusion the ability of spatial learning and memory in PTSD rats is reduced by inhibiting the CBS/H_2S system in hippocampal area. The mechanism of H2S improving spatial learning and memory ability of PTSD rats may be related to the increase of firing frequency and excitability of hippocampal neurons. The ability of spatial learning and memory was decreased by CBS/H_2S system in hippocampal area. The mechanism of H2S improving spatial learning and memory ability of PTSD rats may be related to the increase of firing frequency and excitability of hippocampal neurons.
【作者单位】：滨州医学院基础医学院生理学教研室;滨州医学院附属医院院办;滨州医学院医药研究中心;滨州医学院基础医学院生物化学教研室;滨州医学院药理学教研室;
【基金】：山东省自然科学基金资助项目(NoZR2013HL009,ZR2013HL002,ZR2013HL003,ZR2013HL007) 烟台市科技发展计划项目(No2012ZH248) 山东省高校科技计划项目(NoJ12LM03)
【分类号】：R749.5

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